中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2011年
8期
566-570
,共5页
周治来%靳安民%陈银海%闵少雄
週治來%靳安民%陳銀海%閔少雄
주치래%근안민%진은해%민소웅
脊髓损伤%康复训练%骨髓源性神经干细胞%Nogo-A%NgR
脊髓損傷%康複訓練%骨髓源性神經榦細胞%Nogo-A%NgR
척수손상%강복훈련%골수원성신경간세포%Nogo-A%NgR
Spinal cord injury%Rehabilitation%Bone marrow mesenchymal stem cells%Neural stem cells%Nogo-A protein%NgR protein
目的 观察神经干细胞移植联合康复训练对脊髓损伤大鼠Nogo-A及NgR蛋白表达的影响。方法 共选取80只SD大鼠，采用改良Allen法制成大鼠T10节段脊髓损伤模型，将其随机分为联合治疗组、细胞移植组、康复训练组及模型组。联合治疗组及康复训练组于制模后次日给予滚筒训练与跑台训练，每周训练5d；联合治疗组与细胞移植组于造模后第7天时，将体外培养的骨髓源性神经干细胞移植入大鼠受损脊髓中。每周采用BBB评分法对各组大鼠后肢运动功能进行评定；于干细胞移植后第1，3，7天时每组各取5只大鼠处死，采用Western-blot法检测各组大鼠术后不同时间点脊髓中Nogo-A及NgR蛋白表达。结果 从脊髓损伤后第2周开始，发现联合治疗组大鼠BBB评分在各时间点均明显优于其他各组(P＜0.05)，第5周时康复训练组BBB评分明显优于模型组(P＜0.05)。随着时间推移，各组大鼠Nogo-A及NgR蛋白起始均呈现高表达、随后快速下降等特点，以干细胞移植第7天时联合治疗组的降低幅度最显著，细胞移植组次之，康复训练组与模型组间差异无统计学意义(P＞0.05)。结论 康复训练联合神经干细胞移植治疗脊髓损伤大鼠具有协同效应，能进一步抑制受损脊髓中Nogo-A、NgR蛋白表达，促进脊髓损伤大鼠肢体功能恢复。
目的 觀察神經榦細胞移植聯閤康複訓練對脊髓損傷大鼠Nogo-A及NgR蛋白錶達的影響。方法 共選取80隻SD大鼠，採用改良Allen法製成大鼠T10節段脊髓損傷模型，將其隨機分為聯閤治療組、細胞移植組、康複訓練組及模型組。聯閤治療組及康複訓練組于製模後次日給予滾筒訓練與跑檯訓練，每週訓練5d；聯閤治療組與細胞移植組于造模後第7天時，將體外培養的骨髓源性神經榦細胞移植入大鼠受損脊髓中。每週採用BBB評分法對各組大鼠後肢運動功能進行評定；于榦細胞移植後第1，3，7天時每組各取5隻大鼠處死，採用Western-blot法檢測各組大鼠術後不同時間點脊髓中Nogo-A及NgR蛋白錶達。結果 從脊髓損傷後第2週開始，髮現聯閤治療組大鼠BBB評分在各時間點均明顯優于其他各組(P＜0.05)，第5週時康複訓練組BBB評分明顯優于模型組(P＜0.05)。隨著時間推移，各組大鼠Nogo-A及NgR蛋白起始均呈現高錶達、隨後快速下降等特點，以榦細胞移植第7天時聯閤治療組的降低幅度最顯著，細胞移植組次之，康複訓練組與模型組間差異無統計學意義(P＞0.05)。結論 康複訓練聯閤神經榦細胞移植治療脊髓損傷大鼠具有協同效應，能進一步抑製受損脊髓中Nogo-A、NgR蛋白錶達，促進脊髓損傷大鼠肢體功能恢複。
목적 관찰신경간세포이식연합강복훈련대척수손상대서Nogo-A급NgR단백표체적영향。방법 공선취80지SD대서，채용개량Allen법제성대서T10절단척수손상모형，장기수궤분위연합치료조、세포이식조、강복훈련조급모형조。연합치료조급강복훈련조우제모후차일급여곤통훈련여포태훈련，매주훈련5d；연합치료조여세포이식조우조모후제7천시，장체외배양적골수원성신경간세포이식입대서수손척수중。매주채용BBB평분법대각조대서후지운동공능진행평정；우간세포이식후제1，3，7천시매조각취5지대서처사，채용Western-blot법검측각조대서술후불동시간점척수중Nogo-A급NgR단백표체。결과 종척수손상후제2주개시，발현연합치료조대서BBB평분재각시간점균명현우우기타각조(P＜0.05)，제5주시강복훈련조BBB평분명현우우모형조(P＜0.05)。수착시간추이，각조대서Nogo-A급NgR단백기시균정현고표체、수후쾌속하강등특점，이간세포이식제7천시연합치료조적강저폭도최현저，세포이식조차지，강복훈련조여모형조간차이무통계학의의(P＞0.05)。결론 강복훈련연합신경간세포이식치료척수손상대서구유협동효응，능진일보억제수손척수중Nogo-A、NgR단백표체，촉진척수손상대서지체공능회복。
Objective To study the effects of rehabilitation training combined with the transplantation of bone marrow mesenchymal stem cells-derived neural stem cells (BMSC-D-NSCs) on the expression of Nogo-A and NgR protein in rats after spinal cord injury (SCI).Methods The spinal cords of eighty Sprague-Dawley rats were injured using a modified Allen′s impactor (H = 25 mm) at T10. The injured rats were randomly divided into a combination therapy group which was given rehabilitation training and cell transplants, a cell graft group, a rehabilitation training group and a control group. At the 7th day post SCI, BMSC-D-NSCs were transplanted into the injured spinal cords of the rats in the combination therapy and cell graft groups. Hindlimb movement was assessed using the BassoBeattie-Bresnahan (BBB) scale every week, and protein was extracted from the injured spinal cord tissue for Nogo-A and NgR determination by Western blotting at the 1st, 3rd and 7th day after cell transplantation.Results The average BBB score of the rats in the combination therapy group was significantly higher than that of the other groups from 2 weeks post transplantation. The scores in the rehabilitation training group were significantly higher than in the control group from the 5th week post transplantation. Western blotting showed high expression of Nogo-A and NgR protein 24 h post surgery, but these declined with time. For Nogo-A there was a significant difference among the groups at all three time points. In the combination therapy group the expression declined to a minimum by the 7th day. For NgR protein there was no significant difference between the 1st and 3rd day in any group.Conclusions Rehabilitation training combined with BMSC-D-NSC transplantation can have a synergistic effect on functional recovery from SCI. It can down regulate the expression of Nogo-A and NgR protein.
