CAJ | 학술논문

目的观察原花青素处理去细胞牛颈静脉带瓣管道的生物学特性.方法分别比较去细胞处理、原花青素去细胞联合处理、戊二醛处理和新鲜未处理带瓣牛颈静脉管道管壁、瓣膜的厚度、大体形态、组织学特点、含水量、热皱缩温度及断裂强度,并进行可溶性蛋白含量的测定.结果原花青素去细胞联合处理组牛颈静脉带瓣管道较未处理组的管壁、瓣膜厚度[管壁:(0.81±0.17) mm比(0.79 ±0.14) mm,瓣膜:(0.23±0.07) mm比(0.21 ±0.05) mm,P＞0.05]、组织含水量[管壁:(85.30 ±2.13)％比(88.10±2.93)％,瓣膜:(87.30±2.67)％比(91.40±3.41)％,P＞0.05]无明显变化；管壁、瓣膜断裂强度[管壁:(10.32±1.07)N比(6.83±1.03)N,瓣膜:(7.49±0.81)N比(3.21 ±0.57)N,P＜0.05]和热皱缩温度[管壁:(85.30±1.21)℃比(70.40 ±0.32)℃,瓣膜:(87.30±2.67)℃比(70.70±0.61)℃,P＜0.05]明显提高,与戊二醛处理组比较差异无统计学意义(P＞0.05)原花青素去细胞联合处理组可溶性蛋白的含量较未处理组明显减少[管壁:(0.041 ±0.011)％比(0.178 ±0.037)％；瓣膜:(0.096±0.017)％比(0.311 ±0.063)％；P ＜0.05].结论原花青素去细胞联合处理牛颈静脉带瓣管道材料具有较好的生物学特性.
목적 관찰원화청소처리거세포우경정맥대판관도적생물학특성.방법 분별비교거세포처리、원화청소거세포연합처리、무이철처리화신선미처리대판우경정맥관도관벽、판막적후도、대체형태、조직학특점、함수량、열추축온도급단렬강도,병진행가용성단백함량적측정.결과 원화청소거세포연합처리조우경정맥대판관도교미처리조적관벽、판막후도[관벽:(0.81±0.17) mm비(0.79 ±0.14) mm,판막:(0.23±0.07) mm비(0.21 ±0.05) mm,P＞0.05]、조직함수량[관벽:(85.30 ±2.13)％비(88.10±2.93)％,판막:(87.30±2.67)％비(91.40±3.41)％,P＞0.05]무명현변화；관벽、판막단렬강도[관벽:(10.32±1.07)N비(6.83±1.03)N,판막:(7.49±0.81)N비(3.21 ±0.57)N,P＜0.05]화열추축온도[관벽:(85.30±1.21)℃비(70.40 ±0.32)℃,판막:(87.30±2.67)℃비(70.70±0.61)℃,P＜0.05]명현제고,여무이철처리조비교차이무통계학의의(P＞0.05)원화청소거세포연합처리조가용성단백적함량교미처리조명현감소[관벽:(0.041 ±0.011)％비(0.178 ±0.037)％；판막:(0.096±0.017)％비(0.311 ±0.063)％；P ＜0.05].결론 원화청소거세포연합처리우경정맥대판관도재료구유교호적생물학특성.
Objective To evaluate the biological characteristics of decellularized bovine jugular vein conduit fixed with procyanidins.Methods Thickness,appearance,histology,water content,shrinkage temperature,breaking strength and soluble protein level of wall and valve of bovine jugular vein conduit treated with decellularization,decellularization and procyanidins,glutaraldehyde and fresh respectively.Results Thickness [Wall:(0.81 ±0.17) mm vs (0.79 ±0.14) mm,Valve:(0.23 ±0.07) mm vs (0.21 ±0.05) mm,P＞0.05],appearance,histology,water content [Wall:(85.3 ±2.13)％ vs ( 88.1 ± 2.93 ) ％,Valve:( 87.30 ± 2.67) ％ vs (91.40 ± 3.41 ) ％,P ＞ 0.05] of wall and valve of bovine jugular vein conduit treated with decellularization and procyanidins were sinilar to fresh ones ( P ＞0.05).While the shrinkage temperature [Wall:(85.30 ± 1.21 ) ℃ vs (70.40 ±0.32) ℃,Valve:(87.30 ±2.67) ℃ vs (70.70±0.61) ℃,P＜0.05],breakingstrength [Wall:(10.32±1.07) N vs (6.83±1.03) N,Valve:(7.49±0.81) N vs (3.21±0.57) N,P＜0.05] were improved.And soluble protein level were decreased evidently [Wall:( 0.041 ± 0.011 ) ％ vs ( 0.178 ± 0.037 ) ％ ; Valve:(0.096 ±0.017)％ vs (0.311 ±0.063 )％ ; P ＜0.05].Conclusion Decellularized bovine jugular vein conduit fixed with procyanidins have nice biological characteristics.