CAJ | 학술논문

目的观察血管内皮细胞生长因子受体2(VEGFR2)在骨髓间充质干细胞(MSCs)调控神经干细胞(NSCs)增殖与分化中的作用.方法细胞共培养分为5组:NSCs+MSCs、NSCs+MSCs+SU5416(VEGFR2阻断剂)、NSCs+HMVECs、NSCs+HMVECs+SU5416、NSCs+3T3.共培养6 h后,采用逆转录.聚合酶链反应检测各组NSCs标志物nestin、成熟星形胶质细胞标志物GFAP及Notch信号分子Notchl和hesl的表达.结果在MSCs及HMVECs共培养组中nestin的表达较高,当VEGFR2信号通路被阻断后,nestin的表达明显下降,而成熟神经细胞GFAP的表达却明显增加.同时,在MSCs及HMVECs共培养组中,Notchl、hesl的表达也明显高于3T3对照组及VEGFR2信号通路阻断剂组.结论 VEGFR2在MSCs调控NSCs增殖与分化过程中可能起重要作用.
목적 관찰혈관내피세포생장인자수체2(VEGFR2)재골수간충질간세포(MSCs)조공신경간세포(NSCs)증식여분화중적작용.방법 세포공배양분위5조:NSCs+MSCs、NSCs+MSCs+SU5416(VEGFR2조단제)、NSCs+HMVECs、NSCs+HMVECs+SU5416、NSCs+3T3.공배양6 h후,채용역전록.취합매련반응검측각조NSCs표지물nestin、성숙성형효질세포표지물GFAP급Notch신호분자Notchl화hesl적표체.결과 재MSCs급HMVECs공배양조중nestin적표체교고,당VEGFR2신호통로피조단후,nestin적표체명현하강,이성숙신경세포GFAP적표체각명현증가.동시,재MSCs급HMVECs공배양조중,Notchl、hesl적표체야명현고우3T3대조조급VEGFR2신호통로조단제조.결론 VEGFR2재MSCs조공NSCs증식여분화과정중가능기중요작용.
Objective To study the effect of vascular endothelial growth factor receptor 2 (VEG-FR2) on proliferation and differentiation of neural stem cells (NSCs) regulated by mesenchymal stem cells (MSCs). Methods In co-culture experiments, NSCs were co-cultured with MSCs, human microvascular endothelial ceils (HMVECs) or NIH3T3. To exclude the effect of VEGFR2 ,SU5416 (a selective inhibitor of the tyrosine kinase activity of the VEGFR2) was applied in co-culture system. Six h later,the expression of nestin and GFAP was detected by RT-PCR. Results RT-PCR studies revealed that the expression of nestin in NSCs was up-regulated by MSCs and HMVECs, whereas the level was sharply down-regulated when VEGFR2 was blocked,and the expression of GFAP was increased. Determination of Notchl and Hesl in NSCs showed that MSCs and HMVECs promoted the expression of Notchl and Hesl. Conclusion VEGFR2 may play an important role in the proliferation and differentiation of NSCs regulated by MSCs.