CAJ | 학술논문

目的观察蒿甲醚对日本血吸虫成虫总抗氧化能力的影响.方法体外将血吸虫在含蒿甲醚和氯化血红素的培养液内培养24 h后,或体内感染小鼠经蒿甲醚300 mg/kg治疗6～24 h后,测定虫体的总抗氧化能力.结果体外50μmol/L的蒿甲醚与氯化血红素伍用引起雌虫总抗氧化能力明显下降.体内蒿甲醚作用血吸虫6 h,即见雌虫的总抗氧化能力明显下降.体内、体外试验中,蒿甲醚对雄虫的总抗氧化能力均无影响.结论蒿甲醚诱导雌虫总抗氧化能力下降.
목적관찰호갑미대일본혈흡충성충총항양화능력적영향.방법체외장혈흡충재함호갑미화록화혈홍소적배양액내배양24 h후,혹체내감염소서경호갑미300 mg/kg치료6～24 h후,측정충체적총항양화능력.결과체외50μmol/L적호갑미여록화혈홍소오용인기자충총항양화능력명현하강.체내호갑미작용혈흡충6 h,즉견자충적총항양화능력명현하강.체내、체외시험중,호갑미대웅충적총항양화능력균무영향.결론호갑미유도자충총항양화능력하강.
Objective To study the effect of artemether (Art) on total antioxidant capacity (T-AOC) in adult Schistosoma japonicum. Methods In vitro, the T-AOC was determined in five-week old worms incubated without or with Art and/or hemin for 24 h, and the worms were continuously incubated for 96 h, then worm survival was assessed. In vivo, T-AOC was determined in worms freshly recovered from mice 6 - 24 h after treatment with Art 300 mg/kg. Results Throughout 96 h incubation no worms were killed by 50 μmol/L Art or 50 μmol/L hemin alone, but approximatdy 80% of them were killed by Art plus hemin. Addition of reduced glutathione and vitamin E could significantly block the cidal action of the combined treatment. No effect on T-AOC was seen in the worms exposed to Art or heroin alone for 24 h, but the combined treatment led to a pronounced T-AOC reduction in female worms in vitro. Such a drug effect on female worms was demonstrated in vivo. After female worms were exposed to Art for 6 - 24 h in vivo, their T-AOC was significantly reduced by 40% - 64%. However, no drug effect on male worms' T-AOC was observed in vivo and in vitro exposed to Art plus hemin. Conclusion Art-induced T-AOC reduction in female worms may sensitize them to lethal damages of endogenous and exogenous oxygen radicals.