CAJ | 학술논문

目的探讨雌激素与抗雌激素制剂对子宫内膜癌细胞化疗效应的影响.方法采用细胞凋亡ELISA方法检测雌激素、三苯氧胺与阿霉素相互作用后,ER(+)RL-952子宫内膜癌细胞的凋亡水平.结果阿霉素诱发ER(+)RL-952子宫内膜癌细胞产生一定的细胞凋亡水平,雌激素能阻断阿霉素的作用,使细胞凋亡水平下降,呈剂量依赖反应;三苯氧胺也能诱发细胞凋亡,与阿霉素作用后,促进子宫内膜癌细胞凋亡,高于三苯氧胺单一作用产生的细胞凋亡水平,并与三苯氧胺浓度剂量呈正相关.结论雌激素能阻断阿霉素对子宫内膜癌细胞的细胞凋亡作用,而三苯氧胺能促进阿霉素的作用,三苯氧胺与阿霉素的联合应用有助于为子宫内膜癌临床治疗的选择提供依据.
목적탐토자격소여항자격소제제대자궁내막암세포화료효응적영향.방법채용세포조망ELISA방법검측자격소、삼분양알여아매소상호작용후,ER(+)RL-952자궁내막암세포적조망수평.결과아매소유발ER(+)RL-952자궁내막암세포산생일정적세포조망수평,자격소능조단아매소적작용,사세포조망수평하강,정제량의뢰반응;삼분양알야능유발세포조망,여아매소작용후,촉진자궁내막암세포조망,고우삼분양알단일작용산생적세포조망수평,병여삼분양알농도제량정정상관.결론자격소능조단아매소대자궁내막암세포적세포조망작용,이삼분양알능촉진아매소적작용,삼분양알여아매소적연합응용유조우위자궁내막암림상치료적선택제공의거.
Objective:To study the effect of estrogen and tamoxifen on chemotherapeutic sensitivity in ER(+)endometrial carcinoma cells.Methods:DNA fragmentation as the criteria for apoptotic cell death was used to evaluate the value of estrogen,tamoxifen and adriamycin in ER(+)endometrial carcinoma cells.DNA fragmentation was measured with the cell death ELISA.Results:Adriamycin and tamoxifen could induce apoptosis in ER(+)endometrial carcinoma cell.The cell apoptosis level was decreased with the increasing of 17-β-estradiol concentration(P＜0.001)and was inversely proportional to 17-β-estradiol concentration(IgM)(P＜0.01).The cell apoptosis level was increased with the increasing of tamoxifen concentration(P＜0.01)and was also directly proportional to tamoxifen concentration(IgM).Furthermore,the cell apoptosis level was increased significantly after treated with both tamoxifen and adriamycin.Conclusion:Estrogen may block apoptosis induced by adriamycin in ER(+)endometrial carcinoma cell.Tamoxifen can increase the sensitivity of endometrial carcinoma cell to adriamycin.Tamoxifen combined with chemotherapeutic drug may be of significant therapeutic benefit in ER(+)endometrial carcinoma.