CAJ | 학술논문

目的探讨老年女性α2-Hs糖蛋白(AHSG)基因多态性与动脉硬化以及血清骨相关生化指标的关系.方法用ELISA法测定344名住院20～80岁女性患者骨特异性碱性磷酸酶、Ⅰ型胶原羧基末端肽、Ⅰ型胶原氨基末端肽和护骨素的浓度;对患者基因组的DNA样品作限制性内切酶Sac Ⅰ的PCR-RFLP检测,以确定其基因型,全自动生化仪酶法测定血清TC、TG、HDL-C、LDL-C,电极法测定血钙,DXA测定腰椎正位、仰卧侧位腰椎和股骨颈的骨密度(BMD).GG基因型患者做骨组织病理活检,彩色超声诊断仪测定颈动脉中内膜厚度(IMT).结果 (1)老年女性患者AHSG基因CC型、CG型和GG型分布频率分别为59.7%、25.1%和15.2%.不同基因型血脂、血Ca~(2+)和骨生化指标有明显差异;(2)协方差分析显示,正侧位腰椎、股骨颈的BMD和IMT与老年女性CG+GG基因型呈线性正相关趋势;(3)GG型老年女性患者骨组织病理切片和彩超验证了AHSG基因变异与动脉硬化、骨质疏松发生发展的相关性.结论 AHSG基因多态性变异与老年女性动脉硬化和骨质疏松的发病密切相关.
목적 탐토노년녀성α2-Hs당단백(AHSG)기인다태성여동맥경화이급혈청골상관생화지표적관계.방법 용ELISA법측정344명주원20～80세녀성환자골특이성감성린산매、Ⅰ형효원최기말단태、Ⅰ형효원안기말단태화호골소적농도;대환자기인조적DNA양품작한제성내절매Sac Ⅰ적PCR-RFLP검측,이학정기기인형,전자동생화의매법측정혈청TC、TG、HDL-C、LDL-C,전겁법측정혈개,DXA측정요추정위、앙와측위요추화고골경적골밀도(BMD).GG기인형환자주골조직병리활검,채색초성진단의측정경동맥중내막후도(IMT).결과 (1)노년녀성환자AHSG기인CC형、CG형화GG형분포빈솔분별위59.7%、25.1%화15.2%.불동기인형혈지、혈Ca~(2+)화골생화지표유명현차이;(2)협방차분석현시,정측위요추、고골경적BMD화IMT여노년녀성CG+GG기인형정선성정상관추세;(3)GG형노년녀성환자골조직병리절편화채초험증료AHSG기인변이여동맥경화、골질소송발생발전적상관성.결론 AHSG기인다태성변이여노년녀성동맥경화화골질소송적발병밀절상관.
Objective To investigate the distribution of alpha2-HS glycoprotein (AHSG) gene polymorphisms and the relationships of AHSG gene polymorphisms with atherosclerosis as well as serum bone related biochemical mark-era. Methods Blood samples of 344 hospitalized female patients, aged 20 ～ 80 years, were sampled for serum bone alkaline phosphatase, cross-linked N-telopeptide of collagen type Ⅰ, cross-linked C-telopeptide of collagen type Ⅰ , osteoprotegrin and leptin were determined by ELISA. Serum TC,TG and calcium content were detected. Poly-morphism of AHSG gene was detected by polymerase chain reaction fragment length polymorphisms (PCR-RFLP) of restriction enzyme Sac Ⅰ. BMD (Norland XR-36) of the anteroposterior spine (AP), supine lateral spine (Lat) and femoral neck (FN) were measured. Morphological changes in the aorta and bone of type GG patient were detected by pathological microscopy. IMT were measured by color doppler ultrasound equipment(SEQUOIAS12). Results (1) The genotype frequency of CC, CG, and GG were 59.7%, 25.1% and 15.2% respectively in all elderly female patients. There were significant difference in blood lipids, Ca~(2+) and serum bone relative biochemical markers to different AHSG genotypes. (2)There were significant differences in the BMD of the AP, Lat, FN and IMT and the serum biochemical markers among the CC, CG and GG genotypes. (3)GG-female patients bone tissue pathology section verify the AHSG polymorphism genetic mutation and atherosclerosis, osteoporosis development of the relationship. Conclusion There was close relationship among AHSG polymorphism variation and the incidence of arteriosclerosis and osteoporosis in elderly female.