中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2010年
1期
15-18
,共4页
刘斌%吴孟海%董静%刘宁%李建民%张晋霞%李世英%王瑞敏%陈贵良
劉斌%吳孟海%董靜%劉寧%李建民%張晉霞%李世英%王瑞敏%陳貴良
류빈%오맹해%동정%류저%리건민%장진하%리세영%왕서민%진귀량
脂肪组织%基质细胞%神经元细胞%细胞分化
脂肪組織%基質細胞%神經元細胞%細胞分化
지방조직%기질세포%신경원세포%세포분화
背景:寻找合适的种子细胞是移植治疗脑血管疾病及其他中枢神经系统疾病的关键.目的:观察人脂肪组织来源的基质细胞分化为神经元细胞的能力.方法:脂肪组织取自要求去除腹部多余脂肪的健康成人,供者无传染性疾病和内分泌疾病.分离培养脂肪组织来源的基质细胞,采用神经诱导培养基加GM1对其进行诱导培养.倒置相差显微镜下连续观察细胞生长情况和形态变化,免疫组织化学法鉴定神经前体细胞的特异性标志神经巢蛋白、神经元特异性烯醇化酶和微管联合蛋白2的表达情况.结果与结论:①经神经诱导培养基加GM1诱导后,分化后的细胞大部分呈典型的神经元样细胞形态.②倒置相差显微镜下可见,于诱导后1 h出现神经巢蛋白表达阳性,5 h出现神经元特异性烯醇化酶和微管联合蛋白2表达阳性.提示脂肪基质细胞可分化为神经元细胞,分化后的神经元细胞具有表达神经巢蛋白、神经元特异性烯醇化酶和微管联合蛋白2的功能.
揹景:尋找閤適的種子細胞是移植治療腦血管疾病及其他中樞神經繫統疾病的關鍵.目的:觀察人脂肪組織來源的基質細胞分化為神經元細胞的能力.方法:脂肪組織取自要求去除腹部多餘脂肪的健康成人,供者無傳染性疾病和內分泌疾病.分離培養脂肪組織來源的基質細胞,採用神經誘導培養基加GM1對其進行誘導培養.倒置相差顯微鏡下連續觀察細胞生長情況和形態變化,免疫組織化學法鑒定神經前體細胞的特異性標誌神經巢蛋白、神經元特異性烯醇化酶和微管聯閤蛋白2的錶達情況.結果與結論:①經神經誘導培養基加GM1誘導後,分化後的細胞大部分呈典型的神經元樣細胞形態.②倒置相差顯微鏡下可見,于誘導後1 h齣現神經巢蛋白錶達暘性,5 h齣現神經元特異性烯醇化酶和微管聯閤蛋白2錶達暘性.提示脂肪基質細胞可分化為神經元細胞,分化後的神經元細胞具有錶達神經巢蛋白、神經元特異性烯醇化酶和微管聯閤蛋白2的功能.
배경:심조합괄적충자세포시이식치료뇌혈관질병급기타중추신경계통질병적관건.목적:관찰인지방조직래원적기질세포분화위신경원세포적능력.방법:지방조직취자요구거제복부다여지방적건강성인,공자무전염성질병화내분비질병.분리배양지방조직래원적기질세포,채용신경유도배양기가GM1대기진행유도배양.도치상차현미경하련속관찰세포생장정황화형태변화,면역조직화학법감정신경전체세포적특이성표지신경소단백、신경원특이성희순화매화미관연합단백2적표체정황.결과여결론:①경신경유도배양기가GM1유도후,분화후적세포대부분정전형적신경원양세포형태.②도치상차현미경하가견,우유도후1 h출현신경소단백표체양성,5 h출현신경원특이성희순화매화미관연합단백2표체양성.제시지방기질세포가분화위신경원세포,분화후적신경원세포구유표체신경소단백、신경원특이성희순화매화미관연합단백2적공능.
BACKGROUND: Appropriate seed cell is important for transplantation in the treatment of cerebrovascular disease and other central nervous system disease.OBJECTIVE: To investigate the capacity of human adipose tissue-derived stromal cells (ADSCs) to differentiate into neurons.METHODS: The fatty tissue was harvested from removed abdominal unnecessary fat of healthy adult with no communicable disease or endocrine disease. Human ADSCs were isolated from human liposuction tissues and cultured in neural induction medium with GM1. Invert phase-contrast microscopy was used to observe morphology changes of ADSCs. The expression of nestin, neuron specific enolase (NSE) and microtubule-associated protein 2 (MAP2) were identified by immunocytochemistry.RESULTS AND CONCLUSION: The majority of cells displayed typical appearance of neuronal-like cells following induction. Following 1 hours of induction, some cells began to express nestin, and NSE and MAP2-positive cells were observed at 5 hours. ADSCs can differentiate into neurons, and the differentiated neurons have the capacity of expressing nestin, NSE and MAP2.
