Inhibiting severe acute respiratory syndrome-associated coronavirus by small interfering RNA
  • 万方数据
    中华医学杂志(英文版) 중화의학잡지(영문판)
    CHINESE MEDICAL JOURNAL
    2003年 8期 1262-1264 ,共3页

    张仁礼%郭中敏%陆家海%孟锦绣%周灿权%詹希美%黄冰%余新炳%黄民%潘兴华%凌文华%陈系古%万卓越%郑焕英%鄢心革%王一飞%冉延超%刘新健%马俊鑫%王承宇%张必良

    장인례%곽중민%륙가해%맹금수%주찬권%첨희미%황빙%여신병%황민%반흥화%릉문화%진계고%만탁월%정환영%언심혁%왕일비%염연초%류신건%마준흠%왕승우%장필량



    small interfering RNA%severe acute respiratory syndrome%coronavirus%cytopathic effect,viral


    Objective To evaluate the effectiveness of small interfering RNA (siRNA) on inhibiting severe acute respiratory syndrome (SARS)-associated coronavirus replication, and to lay bases for the future clinical application of siRNA for the treatment of viral infectious diseases.Methods Vero-E6 cells was transfected with siRNA before SARS virus infection, and the effectiveness of siRNA interference was evaluated by observing the cytopathic effect (CPE) on Vero-E6 cells.Results Five pairs of siRNA showed ability to reduce CPE dose dependently, and two of them had the best effect. Conclusion siRNA may be effective in inhibiting SARS-associated coronavirus replication.
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