CAJ | 학술논문

目的运用抗体芯片筛选胰腺癌与癌旁正常胰腺组织之间差异表达的蛋白质,以期发现有价值的新的胰腺癌肿瘤标志物.方法应用含有378种已知蛋白质的单克隆抗体芯片比较7例胰腺导管腺癌混合样品及其配对癌旁正常胰腺组织混合样品的差异表达蛋白谱.结果发现20种在胰腺癌中明显差异表达的蛋白质,其中高表达的蛋白质11种(UbcH6、GABA b R2、Plakophilin 2a、Inhibitor2、Nestin、SheC、PRK2、Neurogenin 3、STAT 3、NHE-3和SRP54),低表达蛋白质9种(DCC、HPV-16 L1、RACKl、Gelsolin、Rabaptin-5、DBP2、IKKa/1、c-Cbl、FXR2).结论抗体芯片是比较蛋白质组学研究的一种有效手段,这些异常表达的蛋白质有助于认识胰腺癌的发病机制,有助于寻找胰腺癌新的诊断标志物和治疗的靶标.
목적 운용항체심편사선이선암여암방정상이선조직지간차이표체적단백질,이기발현유개치적신적이선암종류표지물.방법 응용함유378충이지단백질적단극륭항체심편비교7례이선도관선암혼합양품급기배대암방정상이선조직혼합양품적차이표체단백보.결과 발현20충재이선암중명현차이표체적단백질,기중고표체적단백질11충(UbcH6、GABA b R2、Plakophilin 2a、Inhibitor2、Nestin、SheC、PRK2、Neurogenin 3、STAT 3、NHE-3화SRP54),저표체단백질9충(DCC、HPV-16 L1、RACKl、Gelsolin、Rabaptin-5、DBP2、IKKa/1、c-Cbl、FXR2).결론 항체심편시비교단백질조학연구적일충유효수단,저사이상표체적단백질유조우인식이선암적발병궤제,유조우심조이선암신적진단표지물화치료적파표.
Objective By proteomic analysis of differentially expressed protein profiling in pancreatic cancer using antibody microarray, new tumor marker of pancreatic cancer was supposed to be discovered. Methods The antibody microarray containing 378 monoclonal antibodies was applied to detect the differentially expressed proteins of 7 sets of pancreatic ductal adenocareinoma pooled samples and their paired normal pancreas tissues pooled samples. Results 11 up-regulated proteins (UbcH6, GABA b R2, Plakophilin 2a, Inhibitor 2, Nestin, ShcC, PRK2, Neurogenin 3, STAT 3, NHE-3 and SRP54) and 9 down- regulated proteins (DCC, HPV-16 L1, RACKI, Gelsolin, Rabaptin-5, DBP2, IKKa/I, c-Cbl, FXR2) were found in pancreatic cancer. Conclusions Antibody microarray was an effective comparative proteomic technology. These dys-regulated proteins facilitated to elucidate the mechanisms of pancreatic cancer and to identify new diagnostic markers and therapeutic targets.