中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2008年
5期
311-313
,共3页
尖锐湿疣%T淋巴细胞%细胞因子类%Foxp3%细胞毒T细胞相关抗原4%程序性死亡1
尖銳濕疣%T淋巴細胞%細胞因子類%Foxp3%細胞毒T細胞相關抗原4%程序性死亡1
첨예습우%T림파세포%세포인자류%Foxp3%세포독T세포상관항원4%정서성사망1
Condylomata acuminata%T-lymphocytes%Cytokines%Foxp3%Cytotoxic T lymphocyte associated antigen-4%Programmed death-1
目的 探讨外周血CD4+CD25+调节性T细胞(Treg细胞)表型和功能改变所致的细胞免疫抑制效应在尖锐湿疣发病机制中的作用.方法 采用免疫荧光标染技术,经流式细胞仪检测46例尖锐湿疣患者和43例正常人外周血CD4+CD25+T细胞转录因子Foxp3及细胞毒T细胞相关抗原4(CTLA-4)、糖皮质激素诱导的肿瘤坏死因子受体家族相关基因(GITR)、程序性死亡1(PD-1)等抑制性膜分子的表达水平.同时,用免疫磁珠细胞分选技术从外周血中分选出高纯度的CD4+CD25+T细胞,经体外刺激后分析胞内分泌白介素10(IL-10)和转化生长因子β(TGF-β)阳性细胞数.结果 尖锐湿疣患者外周血CD4+CD25+Foxp3+Treg细胞数量比正常人对照组显著增加(P＜0.01),CD4+CD25+T细胞表面抑制性膜分子CTLA-4、PD-1表达明显增强(P分别为＜0.05及＜0.01),胞内TGF-β阳性细胞增多(P＜0.001).结论 人乳头瘤病毒感染可诱导CD4+CD25+Treg细胞活化增殖,高表达负性共刺激分子和分泌抑制性细胞因子,通过多种机制抑制机体的抗病毒免疫应答.
目的 探討外週血CD4+CD25+調節性T細胞(Treg細胞)錶型和功能改變所緻的細胞免疫抑製效應在尖銳濕疣髮病機製中的作用.方法 採用免疫熒光標染技術,經流式細胞儀檢測46例尖銳濕疣患者和43例正常人外週血CD4+CD25+T細胞轉錄因子Foxp3及細胞毒T細胞相關抗原4(CTLA-4)、糖皮質激素誘導的腫瘤壞死因子受體傢族相關基因(GITR)、程序性死亡1(PD-1)等抑製性膜分子的錶達水平.同時,用免疫磁珠細胞分選技術從外週血中分選齣高純度的CD4+CD25+T細胞,經體外刺激後分析胞內分泌白介素10(IL-10)和轉化生長因子β(TGF-β)暘性細胞數.結果 尖銳濕疣患者外週血CD4+CD25+Foxp3+Treg細胞數量比正常人對照組顯著增加(P＜0.01),CD4+CD25+T細胞錶麵抑製性膜分子CTLA-4、PD-1錶達明顯增彊(P分彆為＜0.05及＜0.01),胞內TGF-β暘性細胞增多(P＜0.001).結論 人乳頭瘤病毒感染可誘導CD4+CD25+Treg細胞活化增殖,高錶達負性共刺激分子和分泌抑製性細胞因子,通過多種機製抑製機體的抗病毒免疫應答.
목적 탐토외주혈CD4+CD25+조절성T세포(Treg세포)표형화공능개변소치적세포면역억제효응재첨예습우발병궤제중적작용.방법 채용면역형광표염기술,경류식세포의검측46례첨예습우환자화43례정상인외주혈CD4+CD25+T세포전록인자Foxp3급세포독T세포상관항원4(CTLA-4)、당피질격소유도적종류배사인자수체가족상관기인(GITR)、정서성사망1(PD-1)등억제성막분자적표체수평.동시,용면역자주세포분선기술종외주혈중분선출고순도적CD4+CD25+T세포,경체외자격후분석포내분비백개소10(IL-10)화전화생장인자β(TGF-β)양성세포수.결과 첨예습우환자외주혈CD4+CD25+Foxp3+Treg세포수량비정상인대조조현저증가(P＜0.01),CD4+CD25+T세포표면억제성막분자CTLA-4、PD-1표체명현증강(P분별위＜0.05급＜0.01),포내TGF-β양성세포증다(P＜0.001).결론 인유두류병독감염가유도CD4+CD25+Treg세포활화증식,고표체부성공자격분자화분비억제성세포인자,통과다충궤제억제궤체적항병독면역응답.
Objective To investigate the possible roles of cellular immunosuppression induced by phenotypic and functional changes of peripheral CD4+CD25+ regulatory T cells (Tregs) in the pathogenesis of condylomata acuminata. Methods Three-color flow cytometry was performed to examine the expression of transcription factor Foxp3 in, along with several inhibitory membrane molecules, i.e. cytotoxic T lympho-cyte associated antigen-4 (CTLA-4), glucocorticoid-induced TNF receptor family-related gene (GITR) and programmed death-1 (PD-1) on peripheral CD4+CD25+ T cells from 46 patients with condylomata acuminata and 43 normal human controls. Meanwhile, high purity of CD4+CD25+ T cells were isolated from peripheral blood using imrnunomagnetic beads, and stimulated to produce intracellular suppressor cytokines such as interleukin-10 (IL-10) and transforming growth factor-beta (TGF-beta), which were detected by flow tyro-metric analysis. Results The number of peripheral CD4+CD25+ Foxp3+ Tregs increased significantly in patients with condylomata acuminata than that in the normal controls (7.37% ± 2.43% vs 5.96% ± 2.09%,P ＜ 0.001). The expressions of CTLA-4 and PD-1 were 1.86% ± 1.13% and 2.41% ± 1.12%, respectively,in the patients, which were significantly higher than those in the normal controls(1.36% ± 0.90% and 1.70%± 0.97%, P ＜ 0.05 and 0.01, respectively). The number of TGF-beta-positive CD4+CD25+ T ceils from peripheral blood were increased in patients than that in, the controls (1.57% ± 0.91% vs 0.78% ± 0.24%, P ＜0.001). Conclusions Human papillomavirus infection can induce the activation and proliferation of CD4+CD25+ Tregs, enhance the expression of negative costimulatory molecules and secretion of suppressor cytokines, and inhibit antiviral immune response through multiple mechanisms.
