中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2011年
10期
1022-1025
,共4页
诸葛小玲%崔大伟%吴英萍%郑书发%靳淼%段招军%余斐%许世佳%陈凌晓%陈瑜
諸葛小玲%崔大偉%吳英萍%鄭書髮%靳淼%段招軍%餘斐%許世佳%陳凌曉%陳瑜
제갈소령%최대위%오영평%정서발%근묘%단초군%여비%허세가%진릉효%진유
杯状病毒%诺如病毒%札如病毒%基因型别
杯狀病毒%諾如病毒%札如病毒%基因型彆
배상병독%낙여병독%찰여병독%기인형별
Human caliciviruses%Norovirus%Sapovirus%Genotype
目的 了解2009-2010年杭州地区急性腹泻患者中杯状病毒基因型别和分子流行病学特征.方法 收集2009-2010年杭州地区920例急性腹泻患者的粪便标本和流行病学资料,用多重PCR方法进行杯状病毒检测,测定部分标本的阳性扩增片段基因序列,并对序列进行系统进化分析.结果 急性腹泻患者杯状病毒检出率为21.8％(201/920),其中诺如病毒(NV)G Ⅰ型25例、GⅡ型170例、札如病毒(SAV) 11例,4例为NVⅠ型和Ⅱ型混合感染、1例为NVⅡ型和SAV混合感染.NV基因型别包括:GⅠ-1(3株)、G Ⅰ-2(1株)、GⅡ-4/2006b变异株(7株)、GⅡ-2(1株)、GⅡ-7(1株)和GⅡ-4/2008变异株(2株);SAV基因型别包括:G Ⅰ -2(5株)、GⅠ-1(4株)和GⅡ-1(1株).杯状病毒的流行在不同季节、年龄组人群均有发病.结论 杯状病毒是2009-2010年引起杭州地区急性腹泻的主要病原之一,其病原具有病毒多样性和基因型别多样性,NVGⅡ-4/2006b变异株或类似株可能是2009-2010年杭州地区流行的优势株.
目的 瞭解2009-2010年杭州地區急性腹瀉患者中杯狀病毒基因型彆和分子流行病學特徵.方法 收集2009-2010年杭州地區920例急性腹瀉患者的糞便標本和流行病學資料,用多重PCR方法進行杯狀病毒檢測,測定部分標本的暘性擴增片段基因序列,併對序列進行繫統進化分析.結果 急性腹瀉患者杯狀病毒檢齣率為21.8％(201/920),其中諾如病毒(NV)G Ⅰ型25例、GⅡ型170例、札如病毒(SAV) 11例,4例為NVⅠ型和Ⅱ型混閤感染、1例為NVⅡ型和SAV混閤感染.NV基因型彆包括:GⅠ-1(3株)、G Ⅰ-2(1株)、GⅡ-4/2006b變異株(7株)、GⅡ-2(1株)、GⅡ-7(1株)和GⅡ-4/2008變異株(2株);SAV基因型彆包括:G Ⅰ -2(5株)、GⅠ-1(4株)和GⅡ-1(1株).杯狀病毒的流行在不同季節、年齡組人群均有髮病.結論 杯狀病毒是2009-2010年引起杭州地區急性腹瀉的主要病原之一,其病原具有病毒多樣性和基因型彆多樣性,NVGⅡ-4/2006b變異株或類似株可能是2009-2010年杭州地區流行的優勢株.
목적 료해2009-2010년항주지구급성복사환자중배상병독기인형별화분자류행병학특정.방법 수집2009-2010년항주지구920례급성복사환자적분편표본화류행병학자료,용다중PCR방법진행배상병독검측,측정부분표본적양성확증편단기인서렬,병대서렬진행계통진화분석.결과 급성복사환자배상병독검출솔위21.8％(201/920),기중낙여병독(NV)G Ⅰ형25례、GⅡ형170례、찰여병독(SAV) 11례,4례위NVⅠ형화Ⅱ형혼합감염、1례위NVⅡ형화SAV혼합감염.NV기인형별포괄:GⅠ-1(3주)、G Ⅰ-2(1주)、GⅡ-4/2006b변이주(7주)、GⅡ-2(1주)、GⅡ-7(1주)화GⅡ-4/2008변이주(2주);SAV기인형별포괄:G Ⅰ -2(5주)、GⅠ-1(4주)화GⅡ-1(1주).배상병독적류행재불동계절、년령조인군균유발병.결론 배상병독시2009-2010년인기항주지구급성복사적주요병원지일,기병원구유병독다양성화기인형별다양성,NVGⅡ-4/2006b변이주혹유사주가능시2009-2010년항주지구류행적우세주.
Objective To investigate the molecular-epidemiologic characteristics and genotypes of human calicivirus (HuCVs) in acute diarrhea patients in Hangzhou from 2009 to 2010.Methods Epidemiologic data and fecal specimens were collected from patients with acute diarrhea.HuCVs of 920 specimens were detected by PCR.PCR products of several positive samples were randomly selected and sequenced.All the sequences were analyzed,phylogenetically.Results 201HuCVs positive cases were identified from 920 facal specimens (21.8％).25 isolates would include norovims G Ⅰ -type,G Ⅱ -type for 170 strains and sapovirus for 1 1 strains.Norovirus G Ⅰ -type and G Ⅱ -type were detected in four specimens at the same time.Other specimens were mixed infection with norovirus G Ⅱ -type and sapovirus.Genotypes of HuCVs showed that norovirus G Ⅰ subtypes were G Ⅰ -1 (3 strains) and G Ⅰ -2 (1 strain).Norovirus G Ⅱ subtypes were G Ⅱ -4/2006b variant strains (7 strains),GⅡ-2 (1 strain),G Ⅱ -7 (1 strain) and G Ⅱ -4/2008 variant strains (2 strains) ;Sapovirus subtypes were G Ⅰ -2 (5 strains),G Ⅰ -1 (4 strains) and G Ⅱ-1 ( 1 strain).The prevalence rates of HuCVs were different in seasons and age groups.Conclusion HuCVs were one of the major pathogens causing acute diarrhea.Both multiple viruses and genotypes of HuCVs were found in the specimens.G Ⅱ-4/2006b variant and similar strains were identified,probably as the prevalent strains from 2009 to 2010 in Hangzhou,Zhejiang province.
