中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2009年
3期
227-231
,共5页
隋军%高伟%李晓江%马静%任艳鑫%宋鑫%肖春杰%唐文如
隋軍%高偉%李曉江%馬靜%任豔鑫%宋鑫%肖春傑%唐文如
수군%고위%리효강%마정%임염흠%송흠%초춘걸%당문여
鼻咽肿瘤%癌,鳞状细胞%基因,bcl-1%多态性,单核苷酸%疾病遗传易感性%预后
鼻嚥腫瘤%癌,鱗狀細胞%基因,bcl-1%多態性,單覈苷痠%疾病遺傳易感性%預後
비인종류%암,린상세포%기인,bcl-1%다태성,단핵감산%질병유전역감성%예후
Nasopharyngeal neoplasms%Carcinoma,squamous cell%Genes,bcl-1%Polymorphism,single nucleotide%Genetic predisposition to disease%Prognosis
目的 研究细胞周期素D1(cyclin DI gene,CCND1)G870A单核苷酸多态性与鼻咽癌患病风险性的关系,并探讨各基因型与鼻咽癌预后的相关性.方法 应用聚合酶链反应-限制性片段长度多态性及测序方法 对241例云南籍汉族鼻咽癌患者和272例云南籍汉族健康对照进行基因分型,计算各基因型与鼻咽癌发病风险以及基因.环境交互作用.结果 CCND1 870A等位基因增加鼻咽癌的发病风险,与GG基因型相比,携带AA、AG基因型个体罹患鼻咽癌比值比(OR)分别为4.79(95%可信限CI为2.77～8.28,P<0.001)和1.72(95%CI为1.10～2.68,P=0.017);至少携带一个870A等位基因的个体罹患鼻咽癌的OR值为2.40(95%C/为1.59～3.63,P<0.001).吸烟与CCND1G870A多态性存在相乘交互作用.分别携带AA、AG、GG基因型患者5年生存率各为56.2%、78.5%、81.4%,AA与GG基因型患者生存率比较P=0.003,AA与AG基因型比较P=0.012,差异均有统计学意义;AG与GG基因型比较P=0.132,差异没有统计学意义.多因素Cox回归模型显示基因型并非影响鼻咽癌预后的独立因素(P=0.501).结论 CCND1 G870A多态性与云南汉族鼻咽癌遗传易感相关,870A等位基因与其预后亦相关.
目的 研究細胞週期素D1(cyclin DI gene,CCND1)G870A單覈苷痠多態性與鼻嚥癌患病風險性的關繫,併探討各基因型與鼻嚥癌預後的相關性.方法 應用聚閤酶鏈反應-限製性片段長度多態性及測序方法 對241例雲南籍漢族鼻嚥癌患者和272例雲南籍漢族健康對照進行基因分型,計算各基因型與鼻嚥癌髮病風險以及基因.環境交互作用.結果 CCND1 870A等位基因增加鼻嚥癌的髮病風險,與GG基因型相比,攜帶AA、AG基因型箇體罹患鼻嚥癌比值比(OR)分彆為4.79(95%可信限CI為2.77～8.28,P<0.001)和1.72(95%CI為1.10～2.68,P=0.017);至少攜帶一箇870A等位基因的箇體罹患鼻嚥癌的OR值為2.40(95%C/為1.59～3.63,P<0.001).吸煙與CCND1G870A多態性存在相乘交互作用.分彆攜帶AA、AG、GG基因型患者5年生存率各為56.2%、78.5%、81.4%,AA與GG基因型患者生存率比較P=0.003,AA與AG基因型比較P=0.012,差異均有統計學意義;AG與GG基因型比較P=0.132,差異沒有統計學意義.多因素Cox迴歸模型顯示基因型併非影響鼻嚥癌預後的獨立因素(P=0.501).結論 CCND1 G870A多態性與雲南漢族鼻嚥癌遺傳易感相關,870A等位基因與其預後亦相關.
목적 연구세포주기소D1(cyclin DI gene,CCND1)G870A단핵감산다태성여비인암환병풍험성적관계,병탐토각기인형여비인암예후적상관성.방법 응용취합매련반응-한제성편단장도다태성급측서방법 대241례운남적한족비인암환자화272례운남적한족건강대조진행기인분형,계산각기인형여비인암발병풍험이급기인.배경교호작용.결과 CCND1 870A등위기인증가비인암적발병풍험,여GG기인형상비,휴대AA、AG기인형개체리환비인암비치비(OR)분별위4.79(95%가신한CI위2.77～8.28,P<0.001)화1.72(95%CI위1.10～2.68,P=0.017);지소휴대일개870A등위기인적개체리환비인암적OR치위2.40(95%C/위1.59～3.63,P<0.001).흡연여CCND1G870A다태성존재상승교호작용.분별휴대AA、AG、GG기인형환자5년생존솔각위56.2%、78.5%、81.4%,AA여GG기인형환자생존솔비교P=0.003,AA여AG기인형비교P=0.012,차이균유통계학의의;AG여GG기인형비교P=0.132,차이몰유통계학의의.다인소Cox회귀모형현시기인형병비영향비인암예후적독립인소(P=0.501).결론 CCND1 G870A다태성여운남한족비인암유전역감상관,870A등위기인여기예후역상관.
Objective The aim of this study was to investigate the susceptibility and prognostic implications of the cyclin D1 gene(CCND1) G870A polymorphism to nasopharyngeal carcinomas (NPC) in Han population in Yunnan China. Methods Two hundred and forty one cases with NPC and 271 matched cancer-free controls were genotyped for the CCND1 G870A polymorphism by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) and sequencing. The adjusted odds ratios (OR) and 95% confidence intervals (CI) were calculated by using unconditional logistic regression model. Overall survival was assessed using univariate and multivariate analyses. Results Contrast with homozygous CCND1 G870G, A allele significantly increasing risk of NPC was associated with homozygous A870A (OR =4.79, 95% CI 2.77 - 8.28, P < 0.001) and heterozygous A870G (OR = 1.72, 95% CI 1.10 - 2.68, P = 0.017). The subjects at least having one CCND1 870A allele had OR of 2.40(95% CI 1.59 -3.63, P < 0.001). Furthermore, smoking may increase the risk of developing NPC interacting with CCND1 G870A polymorphism. Kaplan-Meier analysis and Cox regression analysis demonstrated that the five-year survival rate of subjects with A.A, AG and GG genotypo was 56.2%, 78.5% and 81.4% (AA vs GG, P=0.003; AA vs AG, P =0.012; AG vs GG, P =0.132), but not independent prognostic factor in NPC(P = 0.501). Conclusions The CCND1 870A allele is associated with the NPC in Han population in Yunnan China, meanwhile, showed a significant prognosis for those patients.
