CAJ | 학술논문

目的研究附红细胞体对人和小鼠红细胞的影响,以探讨附红细胞体的感染机制.方法采集5例感染附红细胞体患者静脉血,采用PCR法检测附红细胞体特异片段,并通过流式细胞术检测其红细胞CD35(CR1受体)表达.分离纯化人附红细胞体,以尾静脉注射方式人工感染小鼠,感染后通过血涂片光学显微镜检查,PCR检测及透射电子显微镜检测鉴定感染成功,流式细胞术检测其红细胞CD35表达,同时比较人和小鼠的红细胞计数、Hb含量、红细胞压积和超氧化物歧化酶(SOD)等血液指标变化.数据行t检验.结果感染组小鼠均被附红细胞体感染,感染率在80%以上.感染附红细胞体患者和感染组小鼠血样中PCR检测均出现801 bp特异性片段,而健康对照者和对照组小鼠的血液样本未扩增出特异性片段.感染附红细胞体患者的红细胞CD35表达增加(t=20.96,P<0.01),而小鼠的红细胞CD35并不表达,感染附红细胞体的人和小鼠红细胞数量都明显减少(t=2.58,t=3.08,均P<0.01),Hb含量降低(f=2.38,t=2.78,均P<0.05),红细胞压积略有下降(t=1.56,t=0.11,均P>0.05),SOD活性略有下降(t=0.64,t=1.43,均P>0.05).结论人附红细胞体可以在人和小鼠之间跨种传播,可以破坏红细胞正常结构,附红细胞体能够增加人红细胞CD35的表达,而小鼠的红细胞CD35不表达.
목적 연구부홍세포체대인화소서홍세포적영향,이탐토부홍세포체적감염궤제.방법 채집5례감염부홍세포체환자정맥혈,채용PCR법검측부홍세포체특이편단,병통과류식세포술검측기홍세포CD35(CR1수체)표체.분리순화인부홍세포체,이미정맥주사방식인공감염소서,감염후통과혈도편광학현미경검사,PCR검측급투사전자현미경검측감정감염성공,류식세포술검측기홍세포CD35표체,동시비교인화소서적홍세포계수、Hb함량、홍세포압적화초양화물기화매(SOD)등혈액지표변화.수거행t검험.결과 감염조소서균피부홍세포체감염,감염솔재80%이상.감염부홍세포체환자화감염조소서혈양중PCR검측균출현801 bp특이성편단,이건강대조자화대조조소서적혈액양본미확증출특이성편단.감염부홍세포체환자적홍세포CD35표체증가(t=20.96,P<0.01),이소서적홍세포CD35병불표체,감염부홍세포체적인화소서홍세포수량도명현감소(t=2.58,t=3.08,균P<0.01),Hb함량강저(f=2.38,t=2.78,균P<0.05),홍세포압적략유하강(t=1.56,t=0.11,균P>0.05),SOD활성략유하강(t=0.64,t=1.43,균P>0.05).결론 인부홍세포체가이재인화소서지간과충전파,가이파배홍세포정상결구,부홍세포체능구증가인홍세포CD35적표체,이소서적홍세포CD35불표체.
Objective To evaluate the influence of Eperythrozoon infection on human and mouse erythrocytes and to explore the pathogenesis of Eperythrozoonosis. Methods The specific gene fragment of Eperythrozoon was detected by polymerase chain reaction (PCR) from the venous blood samples of five patients infected with Eperythrozoon. The complement receptor type I (CD35) expression on erythrocytes of these five patients was determined by flow cytometry. Thereafter, the Eperythrozoons were purified from human samples and injected into mice through the tail veins. Blood smear microscopy, PCR and transmission electron microscopy were used to assure the successful infection. The hematological indicators of human and mice, such as red blood cell (RBC) count,hemoglobin (Hb) content, hematocrit and superoxide dismutase (SOD) were evaluated. All results were analyzed by t test. Results More than 80% of treated mice were confirmed to be infected with Eperythrozoon successfully. A fragment of 801 bp specific gene of Eperythrozoon was detected by PCR in samples from both infected patients and infected mice, which were not detected in samples from healthy control people or control mice. CD35 was highly expressed on the erythrocytes of infected patients, but not expressed on the erythrocytes of infected mice. Both RBC counts and Hb content dramatically decreased in infected patients and infected mice. Hematocrit and the activity of SOD also slightly decreased in infected patients and infected mice. Conclusions Eperythrozoon can spread between human and mice and destroy erythrocyte structure. Eperythrozoon can upregulate CD35 expression in human, but there is no CD35 expression in mice.