CAJ | 학술논문

目的探讨正常成人外周血细胞表面磷脂酰丝氨酸(PS)的表达及其在凝血过程中的作用.方法取10名正常成人外周血(每份5ml),分离血小板、中性粒细胞、淋巴细胞和红细胞,用乳黏素和组织因子(TF)抗体标记流式细胞仪检测PS和TF的表达.通过凝血复钙时间和内源性或外源性凝血因子Xa以及凝血酶的生成实验测定各细胞组分的促凝活性,并用乳黏素和TF抗体封闭PS和TF,观察其促凝血拮抗作用.结果正常成人外周血细胞表面有少量PS的表达,阳性率分别为:血小板9.1％、中性粒细胞5.4％、淋巴细胞3.9％、红细胞3.2％；凝血复钙时间试验示血小板、中性粒细胞、淋巴细胞和红细胞分别缩短凝血复钙时间47％、36.5％、25％和12.5％；内源性或外源性凝血因子Xa以及凝血酶生成实验结果显示四种正常血细胞成份使活化酶产量增加13％～26％.结论正常血细胞表面具有不同程度的PS表达,并在体内凝血过程中起到一定的促凝作用.
목적 탐토정상성인외주혈세포표면린지선사안산(PS)적표체급기재응혈과정중적작용.방법 취10명정상성인외주혈(매빈5ml),분리혈소판、중성립세포、림파세포화홍세포,용유점소화조직인자(TF)항체표기류식세포의검측PS화TF적표체.통과응혈복개시간화내원성혹외원성응혈인자Xa이급응혈매적생성실험측정각세포조분적촉응활성,병용유점소화TF항체봉폐PS화TF,관찰기촉응혈길항작용.결과 정상성인외주혈세포표면유소량PS적표체,양성솔분별위:혈소판9.1％、중성립세포5.4％、림파세포3.9％、홍세포3.2％；응혈복개시간시험시혈소판、중성립세포、림파세포화홍세포분별축단응혈복개시간47％、36.5％、25％화12.5％；내원성혹외원성응혈인자Xa이급응혈매생성실험결과현시사충정상혈세포성빈사활화매산량증가13％～26％.결론 정상혈세포표면구유불동정도적PS표체,병재체내응혈과정중기도일정적촉응작용.
Objective To investigate the expression and procoagulant activity of phosphatidylserine (PS) on the normal peripheral blood cells of adults.Methods Normal peripheral blood samples were collected from 10 healthy volunteers (5 ml from each volunteer),platelets,neutrophils,lymphocytes and erythrocytes were isolated.The expression and procoagulant activity of PS on normal blood cells were identified by flow cytometry,inhibition test with lactadherin as PS probe and coagulation anticoagulant,respectively.Results There was PS expression on a few normal blood cells (9.1％,5.4％,3.9％ and 3.2％ in platelets,neutrophils,lymphocytes and erythrocytes,respectively).The PS on these normal blood cells in vitro showed significant procoagulant activity.The plasma recalcification time was shortened by 47％,36.5％,25％ and 12.5％ by platelets,neutrophils,lymphocytes and erythrocytes,respectively; the formation of factor X a (through both intrinsic and extrinsic pathways) and thrombin was also increased by 13％ -26％ by platelets,neutrophils,lymphocytes and erythrocytes,respectively.Conclusion The PS on normal blood cells in vivo may play a crucial role in the coagulation cascade.