CAJ | 학술논문

目的研究胃癌表达胃泌素受体(gastrin receptor,GR)的特征及意义.方法应用受体的放射配基结合分析法测定34例胃癌标本GR含量及其亲和力,并比较胃癌表达的GR与其部位、分期、分级、胃癌细胞DNA含量、邻近正常胃粘膜的GR及患者生存期的关系.结果 34例胃癌组织中,16例(47.1%)胃癌GR阳性,其中14例(41.2%)表达高亲和力GR,2例表达低亲和力GR;在高亲和力GR中,9例表达高含量GR(平均含量39.5±14.4 fmol/mg蛋白),5例表达低含量GR(平均含量6.0±2.8 fmol/mg蛋白).胃体癌(77.8%)和贲门癌(50%)较胃窦癌(20.1%)更易于表达高亲和力GR.相同部位的胃癌及邻近正常粘膜表达GR是一致的,但胃癌表达的胃泌素受体含量(39.5±14.4 fmol/mg蛋白)高于粘膜(26.1±16.6 fmol/mg蛋白).Ⅲ、Ⅳ期胃癌表达高亲和力GR的比例(1 3/24)明显高于Ⅰ、Ⅱ期胃癌(1/10).胃癌表达的GR与癌细胞的分级无关.随访23～61月发现,对于Ⅲ、Ⅳ期胃癌患者,13例表达高亲和力GR的胃癌,有11例死亡;而11例低亲和力GR或GR阴性的胃癌,仅有4例死亡.结论 GR在胃癌自分泌生长过程中也是一个重要的因素;GR有利于预测患者的预后及指导GR拮抗剂的应用.
목적연구위암표체위비소수체(gastrin receptor,GR)적특정급의의.방법 응용수체적방사배기결합분석법측정34례위암표본GR함량급기친화력,병비교위암표체적GR여기부위、분기、분급、위암세포DNA함량、린근정상위점막적GR급환자생존기적관계.결과 34례위암조직중,16례(47.1%)위암GR양성,기중14례(41.2%)표체고친화력GR,2례표체저친화력GR;재고친화력GR중,9례표체고함량GR(평균함량39.5±14.4 fmol/mg단백),5례표체저함량GR(평균함량6.0±2.8 fmol/mg단백).위체암(77.8%)화분문암(50%)교위두암(20.1%)경역우표체고친화력GR.상동부위적위암급린근정상점막표체GR시일치적,단위암표체적위비소수체함량(39.5±14.4 fmol/mg단백)고우점막(26.1±16.6 fmol/mg단백).Ⅲ、Ⅳ기위암표체고친화력GR적비례(1 3/24)명현고우Ⅰ、Ⅱ기위암(1/10).위암표체적GR여암세포적분급무관.수방23～61월발현,대우Ⅲ、Ⅳ기위암환자,13례표체고친화력GR적위암,유11례사망;이11례저친화력GR혹GR음성적위암,부유4례사망.결론 GR재위암자분비생장과정중야시일개중요적인소;GR유리우예측환자적예후급지도GR길항제적응용.
Objective: To investigate the characteristics and significance of gastrin receptor (GR) expressionin gastric cancer. Methods: The content and affinity of GR were determined in 34 specimens of gastriccancer using radioligand binding assay. The correlation was analyzed between GR expression in tumorsand tumor sites, stages, grades, DNA of gastric cancer cells, GR of adjacent normal gastric mucosa, survivaltime. Results: Among the 34 cases of gastric cancer, 16 patients (47.1%) had positive GR in specimens ofgastric cancer, with high-affinity GR in 14 cases (41.2%) and low-affinity GR in 2 cases. Of high-affinityGR, 9 cases had cancers with GR＞10 fmol/mg.protein (39.5±14.4 fmol/mg.protein), 5 cases with GR≤10fmol/mg.protein (6.0±2.8 fmol/mg.protein). High-affinity GR was easier to be expressed in cancers ofgastric body (7/9) and cardia (3/6) than in gastric antrum (4/19). The expression of GR in gastric canceraccorded well with that in normal gastric mucosa at the same sites, but with more high-special binding sitesthan the latter (39.5±14.4 vs 26.1±16.6 fmol/mg.protein). A significantly greater proportion of patientswith Ⅲ+Ⅳ stages (13/24) had high-affinity GR compared with Ⅰ+Ⅱ stages (1/10) of gastric cancers. Duringa follow-up of 23-61 months, 11 of 13 cases with high-affinity GR were dead, whereas 4 of 11 cases withlow-affinity or negative GR were dead in Ⅲ+Ⅳ stages of gastric cancer. Conclusion: GR is an importantfactor in the autocrine growth of gastric cancer cells, and helpful in the prediction of prognosis and guidanceof treatment with GR antagonists.