CAJ | 학술논문

果蝇心脏位于身体背部,是一个体节性重复的线性管状结构.在hedgehog(hh)基因的信号诱导下,seven-up(svp)基因调控果蝇的心脏发育,在每个体节的两个心肌细胞和两个副心肌细胞中表达.结果表明,在svp纯合突变体中,报告基因lacZ在心肌细胞中的表达图式正常,但在副心肌细胞中的表达图型明显异常,而且部分EPC细胞生长尺寸增加.某些体节的DA1肌肉祖细胞缺失,晚期突变体胚胎体壁肌肉细胞也呈现异常,表明基因svp的活性对果蝇副心肌细胞、DA1肌肉祖细胞和体壁肌肉细胞的分化是必须的,并且可能与EPC副心肌细胞的尺寸生长有关.
과승심장위우신체배부,시일개체절성중복적선성관상결구.재hedgehog(hh)기인적신호유도하,seven-up(svp)기인조공과승적심장발육,재매개체절적량개심기세포화량개부심기세포중표체.결과표명,재svp순합돌변체중,보고기인lacZ재심기세포중적표체도식정상,단재부심기세포중적표체도형명현이상,이차부분EPC세포생장척촌증가.모사체절적DA1기육조세포결실,만기돌변체배태체벽기육세포야정현이상,표명기인svp적활성대과승부심기세포、DA1기육조세포화체벽기육세포적분화시필수적,병차가능여EPC부심기세포적척촌생장유관.
The Drosophila dorsal vessel is a segmentally repeated linear organ, in which seven-up (svp) is expressed in two pairs of cardioblasts and two pairs of pericardial cells in each segment. Under the control of hedgehog (hh) signaling from the dorsal ectoderm, svp participates in diversifying cardioblast identities within each segment. In this experiment, the homozygous embryos of svp mutants exhibited an increase in cell size of Eve positive pericardial cells (EPCs) and a disarranged expression pattern, while the cardioblasts pattern of svp-lacZ expression was normal. In the meantime, the DA1 muscle founders were absent in some segments in svp mutant embryos, and the dorsal somatic muscle patterning was also severely damaged in the late stage mutant embryos,suggesting that svp is required for the differentiation of Eve-positive pericardial cells and DA1 muscle founders and may have a role in EPC cell growth.