CAJ | 학술논문

为明确中国北纬33度地区小麦纹枯病病原菌的组成和致病力,对分离自江苏、安徽、河南、湖北4省的157株丝核菌,通过Hoechst 33258荧光染色法进行细胞核数量观测,利用菌丝融合的方法测定其所属融合群,并测定了其中111株丝核菌株对3个小麦品种(CI12633、陕229、扬麦158)麦苗的致病力.结果表明,在供试菌株中,150株为双核丝核菌,占95.54%,都属于AG-D融合群;7株为多核丝核菌,占4.46%,其中3株属于AG-1-IB融合群,3株属于AG11融合群,1株属于AG5融合群.供试菌株的致病力存在明显差异,双核丝核菌的致病力显著高于多核丝核菌,AG-D融合群中两种不同融合频率的菌株致病力无明显差异.不同地区的菌株间致病力无明显差异,菌株对3个小麦品种的致病力有显著差异,对CI12633致病力最强,对陕229致病力最弱,对扬麦158的致病力居中.
위명학중국북위33도지구소맥문고병병원균적조성화치병력,대분리자강소、안휘、하남、호북4성적157주사핵균,통과Hoechst 33258형광염색법진행세포핵수량관측,이용균사융합적방법측정기소속융합군,병측정료기중111주사핵균주대3개소맥품충(CI12633、협229、양맥158)맥묘적치병력.결과표명,재공시균주중,150주위쌍핵사핵균,점95.54%,도속우AG-D융합군;7주위다핵사핵균,점4.46%,기중3주속우AG-1-IB융합군,3주속우AG11융합군,1주속우AG5융합군.공시균주적치병력존재명현차이,쌍핵사핵균적치병력현저고우다핵사핵균,AG-D융합군중량충불동융합빈솔적균주치병력무명현차이.불동지구적균주간치병력무명현차이,균주대3개소맥품충적치병력유현저차이,대CI12633치병력최강,대협229치병력최약,대양맥158적치병력거중.
Wheat sharp eyespot is one of the most important diseases in the north latitude 33° regions of china. One hundred and fifty-seven Rhizoctonia isolates were isolated from different areas of Jiangsu, Anhui, Henan and Hubei province in 2008 and 2009. Identification results showed, among these isolates, 150 isolates were Rhizoctonia cerealis with percentage of 95.54%, and 7 isolates were Rhizoctonia solani with percentage of 4.46%. All R. cerealis isolates were in AG-D anastomosis group. Among the 7 isolates of R. solani, based on anastomosis and blast of their ITS sequences, one isolate was in AG5 anastomosis group, and 3 isolates were in AG11 and 3 isolates were in AG-1-IB, respectively. The virulence of 111 isolates was tested with artificial inoculation method in greenhouse. The results showed that virulence of these isolates was different significantly. The virulence of R. cerealis isolates was higher than that of R. solani isolates. No significant difference was observed between two R. cerealis AG-D groups with different anastomosis frequency. The virulence of isolates from 4 provinces was not significantly different. The virulence of isolates to 3 wheat cultivars was significantly different, and it was the highest to CI12633 and the lowest to Shaan 229.