CAJ | 학술논문

目的研究硒化壳聚糖对急性早幼粒白血病细胞株NB4增殖和凋亡的影响,探讨这种影响与PML-RARα融合蛋白表达的关系.方法应用MTT法检测硒化壳聚糖对细胞增殖的影响,应用AO/EB荧光染色法共聚焦显微镜下观察诱导凋亡作用,应用Western blot的方法检测细胞PML-RARα融合蛋白含量的变化.结果硒化壳聚糖可对体外培养的NB4细胞产生剂量和时间依赖性的抑制作用,50 mg/L 至 100 mg/L硒化壳聚糖作用NB4细胞24 h可诱导细胞出现明显的凋亡现象,经硒化壳聚糖处理24 h后,NB4细胞内PML-RARα融合蛋白含量明显减少.结论硒化壳聚糖可通过下调PML-RARα融合蛋白含量对NB4细胞产生抑制增殖和诱导凋亡作用.
목적 연구서화각취당대급성조유립백혈병세포주NB4증식화조망적영향,탐토저충영향여PML-RARα융합단백표체적관계.방법 응용MTT법검측서화각취당대세포증식적영향,응용AO/EB형광염색법공취초현미경하관찰유도조망작용,응용Western blot적방법검측세포PML-RARα융합단백함량적변화.결과 서화각취당가대체외배양적NB4세포산생제량화시간의뢰성적억제작용,50 mg/L 지 100 mg/L서화각취당작용NB4세포24 h가유도세포출현명현적조망현상,경서화각취당처리24 h후,NB4세포내PML-RARα융합단백함량명현감소.결론 서화각취당가통과하조PML-RARα융합단백함량대NB4세포산생억제증식화유도조망작용.
Objective To investigate the effects of Selenium chiston (Sc) on proliferation and apoptosis of NB4 cells and explore its relationship with PML-RARα fusion proteins.Methods MTT was used to determine the proliferative effects of drugs on NB4 cells and AO/EB fluoresent staining method was used to observe the inducing apoptosis effect of drugs. Western blot was used to examine the abundance of PML-RARα fusion proteins expressed by tumor cells.Results An exposure of NB4 cells to Sc produced both concentration and time-dependent increase in the anti-proliferative rate.Treatment with 50 mg/L to 100 mg/L of Sc for 24 h could induce NB4 cells apoptosis. The abundance of PML-RARα fusion proteins was strongly down-regulated in Sc-treated NB4 cells.Conclusion Selenium chiston can inhibit the proliferation and induce apoptosis of NB4 cells and these effects are correlated with down-regulation of the abundance of PML-RARα fusion proteins.