CAJ | 학술논문

研究了混合溶剂体系中阴离子分别为BF4-,PF6-,Cl-,Br-和r的14种离子液体对来源于黑布林糖苷酶催化合成红景天苷反应的影响.结果表明,在最佳反应条件下,β-糖苷酶的反应初速度和红景天苷的产率分别为3.3mmol/(L·h)和24.5％.离子液体咪唑阳离子的烷基链长(C2～C10)对β-糖苷酶的活性影响较大,当烷基链长为C6时,糖苷酶表现出较高的催化活性.
연구료혼합용제체계중음리자분별위BF4-,PF6-,Cl-,Br-화r적14충리자액체대래원우흑포림당감매최화합성홍경천감반응적영향.결과표명,재최가반응조건하,β-당감매적반응초속도화홍경천감적산솔분별위3.3mmol/(L·h)화24.5％.리자액체미서양리자적완기련장(C2～C10)대β-당감매적활성영향교대,당완기련장위C6시,당감매표현출교고적최화활성.
The preparation of salidroside was successfully carried out in fourteen ionic liquids (ILs)-containing systems using β-glycosidase from black plum seeds for the first time.The optimum conditions were determined for C6Mlm·BF4,pH,phosphate buffer content,and molar ratio of tyrosol to D-glucose to be 1％ (v/v),5.9,20％ (v/v),and 8∶1,under which the initial reaction rate and yield were 3.3 mmol/(L·h) and 24.5％,respectively.Moreover,the effects of 1-alkylimidazolium-based ILs possessing different alkyl chain lengths from C2 to C10 and a variety of anions including BF-4,PF-6,Cl-,Br-,and I- on enzyme activity in co-solvent systems were investigated.The results indicate that the optimal chain length of the alkyl substituent on the imidazolium ring of the cation was C6.