中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2008年
8期
737-739
,共3页
金立达%吴立琴%林丽娜%王万铁
金立達%吳立琴%林麗娜%王萬鐵
금립체%오립금%림려나%왕만철
氟比洛芬酯%呼吸,人工%呼吸窘迫综合征,成人
氟比洛芬酯%呼吸,人工%呼吸窘迫綜閤徵,成人
불비락분지%호흡,인공%호흡군박종합정,성인
Flurbiprofen%Respiration,artificial%Respiratory distress syndrome,adult
目的 探讨氟比洛芬酯对大鼠机械通气所致肺损伤的影响.方法 健康成年雄性SD大鼠40只,体重300～350 g,随机分为4组(n=10),常规潮气量通气组(TV组,潮气量8 ml/ks)、大潮气量通气组(HV组,潮气量40 ml/ks)、大潮气量通气+氟比洛芬酯5 ms/kg组(HV+F1组)和大潮气量通气+氟比洛芬酯10 mg/kg组(HV+F2组).HV+F1组和HV+F2组于机械通气前15 min时分别静脉注射氟比洛芬酯5、10 mg/ks.于机械通气4 h时处死大鼠,测定支气管肺泡灌洗液(BALF)肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、血栓素B2(TXB2)和总蛋白浓度,计数白细胞及计算肺组织湿/干重比(W/D),光镜下观察肺组织病理学结果.结果 与TV组相比,HV组BALF TNF-α、IL-6、TXB2、总蛋白浓度、白细胞计数和肺组织W/D升高(P<0.05),肺组织发生病理学损伤;与HV组相比,HV+F1组和HV+F2组BALF TNF-α、IL-6、TXB2、总蛋白浓度、白细胞计数和肺组织W/D降低(P<0.05),肺组织病理学损伤减轻;HV+F2组BALF TNF-α和TXB2浓度低于HV+F1组(P<0.05),其余指标差异均无统计学意义(P>0.05).结论 氟比洛芬酯可通过抑制炎性反应减轻大鼠机械通气所致肺损伤.
目的 探討氟比洛芬酯對大鼠機械通氣所緻肺損傷的影響.方法 健康成年雄性SD大鼠40隻,體重300～350 g,隨機分為4組(n=10),常規潮氣量通氣組(TV組,潮氣量8 ml/ks)、大潮氣量通氣組(HV組,潮氣量40 ml/ks)、大潮氣量通氣+氟比洛芬酯5 ms/kg組(HV+F1組)和大潮氣量通氣+氟比洛芬酯10 mg/kg組(HV+F2組).HV+F1組和HV+F2組于機械通氣前15 min時分彆靜脈註射氟比洛芬酯5、10 mg/ks.于機械通氣4 h時處死大鼠,測定支氣管肺泡灌洗液(BALF)腫瘤壞死因子α(TNF-α)、白細胞介素6(IL-6)、血栓素B2(TXB2)和總蛋白濃度,計數白細胞及計算肺組織濕/榦重比(W/D),光鏡下觀察肺組織病理學結果.結果 與TV組相比,HV組BALF TNF-α、IL-6、TXB2、總蛋白濃度、白細胞計數和肺組織W/D升高(P<0.05),肺組織髮生病理學損傷;與HV組相比,HV+F1組和HV+F2組BALF TNF-α、IL-6、TXB2、總蛋白濃度、白細胞計數和肺組織W/D降低(P<0.05),肺組織病理學損傷減輕;HV+F2組BALF TNF-α和TXB2濃度低于HV+F1組(P<0.05),其餘指標差異均無統計學意義(P>0.05).結論 氟比洛芬酯可通過抑製炎性反應減輕大鼠機械通氣所緻肺損傷.
목적 탐토불비락분지대대서궤계통기소치폐손상적영향.방법 건강성년웅성SD대서40지,체중300～350 g,수궤분위4조(n=10),상규조기량통기조(TV조,조기량8 ml/ks)、대조기량통기조(HV조,조기량40 ml/ks)、대조기량통기+불비락분지5 ms/kg조(HV+F1조)화대조기량통기+불비락분지10 mg/kg조(HV+F2조).HV+F1조화HV+F2조우궤계통기전15 min시분별정맥주사불비락분지5、10 mg/ks.우궤계통기4 h시처사대서,측정지기관폐포관세액(BALF)종류배사인자α(TNF-α)、백세포개소6(IL-6)、혈전소B2(TXB2)화총단백농도,계수백세포급계산폐조직습/간중비(W/D),광경하관찰폐조직병이학결과.결과 여TV조상비,HV조BALF TNF-α、IL-6、TXB2、총단백농도、백세포계수화폐조직W/D승고(P<0.05),폐조직발생병이학손상;여HV조상비,HV+F1조화HV+F2조BALF TNF-α、IL-6、TXB2、총단백농도、백세포계수화폐조직W/D강저(P<0.05),폐조직병이학손상감경;HV+F2조BALF TNF-α화TXB2농도저우HV+F1조(P<0.05),기여지표차이균무통계학의의(P>0.05).결론 불비락분지가통과억제염성반응감경대서궤계통기소치폐손상.
Objective To investigate the effects of flurbiprofen on ventilator-induced lung injury in rats. Methods Forty healthy adult male SD rats weighing 300-350 g were randomly divided into 4 gorups ( n = 10 each) : group A traditional tidal volume (VT= 8 ml/kg) ; group B high tidal volume (HV, VT = 40 ml/kg) ; group C HV + flurbiprefen 5 ms/kg and group D HV + flurbiprofen 10 ms/kg. The animals were anesthetized with intraperitonel 20% urethane 8 ml/kg, tracheostomized and mechanically ventilated. In group C and D flurbiprofen was given iv at 15 rain before mechanical ventilation. The animals were mechanically ventilated for 4 h and then sacrificed. The lungs were removed for determination of TNF-α, IL-6, TXB2 total protein concentrations and WBC count in bronchoalveolar lavage fluid (BALF), W/D lung weight ratio and microscopic examination. Results The TNF-α, IL-6, TXB2, total protein concentrations, the number of WBCs and W/D ratio were significantly higher and the histologic damage was significantly more serious in group B than in group A. Flurbiprofen significantly attenuated the HV-induced changes in group C and D. The TNF-α, TXB2 concentrations in BALF were significantly lower in group D than in group C. Conclusion Fhrbiprofen can attenuate ventilator-induced lung injury in rats.
