CAJ | 학술논문

对2007年8月至2011年4月在我院行手术治疗的40例肝细胞癌(HCC)患者肝癌组织及其中10例患者的HCC癌旁组织、10例肝血管瘤的肝组织及5例精原细胞瘤组织,采用免疫组化链霉菌抗生物素蛋白-过氧化物酶连结法,行干细胞转录因子Nanog蛋白表达的检测.各类组织中Nanog蛋白表达的比例分别为42％( 17/40)、1/10、0/10和5/5；Nanog蛋白表达与性别、年龄、血清AFP水平、HBsAg阳性、肿瘤分化情况、Child分级及TNM分期,无明显相关性(P＞0.05).Nanog蛋白可作为HCC干细胞的表面标志物.
대2007년8월지2011년4월재아원행수술치료적40례간세포암(HCC)환자간암조직급기중10례환자적HCC암방조직、10례간혈관류적간조직급5례정원세포류조직,채용면역조화련매균항생물소단백-과양화물매련결법,행간세포전록인자Nanog단백표체적검측.각류조직중Nanog단백표체적비례분별위42％( 17/40)、1/10、0/10화5/5；Nanog단백표체여성별、년령、혈청AFP수평、HBsAg양성、종류분화정황、Child분급급TNM분기,무명현상관성(P＞0.05).Nanog단백가작위HCC간세포적표면표지물.
From August 2007 to April 2011,hepatocellular carcinoma (HCC) (n =40),paraHCC tissues (n =10),seminoma (n =10) and cavernous hemangioma (n =10) were selected.And the method of immunohistochemical streptavidin-perosidase was applied to detect the protein expression of Nanog.The expression ratios of Nanog were 17/40 (42％),1/10,0/10 and 5/5 in HCC,para-HCC tissues,seminoma and cavernous hemangioma respectively.Its expression showed no significant correlation with the patient gender,age,serum alpha fetoprotein (AFP),hepatitis B surface antigen (HBsAg),differentiation,Child grade and TNM stage ( P ＞ 0.05 ).It may be used as a surface marker of liver cancer stem cell.