CAJ | 학술논문

目的探讨草酸钙尿结石患者肾组织内维生素K依赖羧化酶(GGCX或VKDC)的表达及意义.方法收集肾脏皮质组织标本,其中草酸钙结石组12例,对照组23例(肾肿瘤皮质组17例,肾盂输尿管交界处狭窄和输尿管结核组6例),应用免疫组织化学法确定GGCX在组织中的定位,应用逆转录.聚合酶链反应(RT-PCR)测定各组间GGCX mRNA表达的差异,应用免疫印迹(Westem blot)蛋白半定量法,对比各组GGCX蛋白的表达水平.结果 GGCX定位于肾皮质肾小管上皮细胞的胞质.结石组GGCX mRNA的表达(0.0799±0.0116)明显低于对照组(0.3887±0.0732和0.3362±0.0694),差异有统计学意义(P<0.05).在蛋白水平上,结石组GGCX的表达(27.64±0.29)明显低于对照组(55.22±0.36和53.78±0.33),差异有统计学意义(P<0.05).结论草酸钙尿结石患者肾组织内GGCX表达降低在草酸钙尿结石形成中有重要作用.
목적 탐토초산개뇨결석환자신조직내유생소K의뢰최화매(GGCX혹VKDC)적표체급의의.방법 수집신장피질조직표본,기중초산개결석조12례,대조조23례(신종류피질조17례,신우수뇨관교계처협착화수뇨관결핵조6례),응용면역조직화학법학정GGCX재조직중적정위,응용역전록.취합매련반응(RT-PCR)측정각조간GGCX mRNA표체적차이,응용면역인적(Westem blot)단백반정량법,대비각조GGCX단백적표체수평.결과 GGCX정위우신피질신소관상피세포적포질.결석조GGCX mRNA적표체(0.0799±0.0116)명현저우대조조(0.3887±0.0732화0.3362±0.0694),차이유통계학의의(P<0.05).재단백수평상,결석조GGCX적표체(27.64±0.29)명현저우대조조(55.22±0.36화53.78±0.33),차이유통계학의의(P<0.05).결론 초산개뇨결석환자신조직내GGCX표체강저재초산개뇨결석형성중유중요작용.
Objective To study the expression of vitamin K dependent gamma-glutamyl carboxyl-ase in patients with urolithiasis. Methods Twelve specimens of renal tissues were harvested from urolithie patients,and control tissues were harvested from renal tumor patients undergoing nephrectomy (17 speci-mens) and non-urolithic patients (6 specimens). Immunohistochemical technique was used to study the location of GGCX in renal tissues. Reverse transcription pelymerase chain reaction (RT-PCR) and West-ern blot techniques were used to detect the differential expression of GGCX mRNA and protein in renal tis-sues. Results GGCX protein located in the cytoplasm of renal tubular epithelial cells. RT-PCR revealed that the expression of GGCX mRNA in urolithic group was (0.0799±0.0116), significantly lower than in the control groups [(0.3887±0.0732) and (0.3362±0.0694), respectively, P < 0.05]. The protein expression of GGCX in urolithic group [(27.64±0.29)] was weaker than in control groups [(55.22±0.36) and (53.78±0.33), respectively, P < 0.05]. Conclusion The low expression of carboxylase inthe calcium oxalate urolithic patients may play an important role in the formation of calcium oxalate urolith-iasis.