中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2009年
29期
2077-2080
,共4页
异丙酚%水通道蛋白-4%星形胶质细胞%水肿
異丙酚%水通道蛋白-4%星形膠質細胞%水腫
이병분%수통도단백-4%성형효질세포%수종
Propofol%Aquaporin-4%Astrocyte%Swelling
目的 探讨异丙酚对氨处理的大鼠脑皮质星形胶质细胞水肿及水通道蛋白-4(AQP4)表达的影响.方法 原代培养SD大鼠脑皮质星形胶质细胞.分别用氯化铵(5 mmol/L)共培养细胞6、12、24、48 h;二甲亚砜及异丙酚(10 μmol/L)预处理细胞30 min后与氯化铵共培养24 h,用蛋白免疫印迹法(Western blot)检测星形胶质细胞AQP4蛋白表达,光学显微镜观察细胞形态,3-4,5-二甲基噻唑-2,5-二酚四唑溴化物(MTT)检测各组细胞活性.结果 氯化铵处理星形胶质细胞12 h后AQP4表达开始上调,一直持续到48 h,其中24 h时AQP4表达上调最为明显,异丙酚预处理组AQP4表达较NH4Cl-24 h组明显降低(P<0.05);光学显微镜观察发现NH4Cl-24 h组细胞较正常组和异丙酚预处理组细胞肿胀明显;MTT检测显示异丙酚预处理组细胞活性较NH4Cl-24 h组和二甲亚砜组明显增高(P<0.05).结论 异丙酚预处理能抑制氯化铵引起的大鼠脑皮质星形胶质细胞AQP4表达的上调,减轻细胞肿胀程度,提高星形胶质细胞活性.
目的 探討異丙酚對氨處理的大鼠腦皮質星形膠質細胞水腫及水通道蛋白-4(AQP4)錶達的影響.方法 原代培養SD大鼠腦皮質星形膠質細胞.分彆用氯化銨(5 mmol/L)共培養細胞6、12、24、48 h;二甲亞砜及異丙酚(10 μmol/L)預處理細胞30 min後與氯化銨共培養24 h,用蛋白免疫印跡法(Western blot)檢測星形膠質細胞AQP4蛋白錶達,光學顯微鏡觀察細胞形態,3-4,5-二甲基噻唑-2,5-二酚四唑溴化物(MTT)檢測各組細胞活性.結果 氯化銨處理星形膠質細胞12 h後AQP4錶達開始上調,一直持續到48 h,其中24 h時AQP4錶達上調最為明顯,異丙酚預處理組AQP4錶達較NH4Cl-24 h組明顯降低(P<0.05);光學顯微鏡觀察髮現NH4Cl-24 h組細胞較正常組和異丙酚預處理組細胞腫脹明顯;MTT檢測顯示異丙酚預處理組細胞活性較NH4Cl-24 h組和二甲亞砜組明顯增高(P<0.05).結論 異丙酚預處理能抑製氯化銨引起的大鼠腦皮質星形膠質細胞AQP4錶達的上調,減輕細胞腫脹程度,提高星形膠質細胞活性.
목적 탐토이병분대안처리적대서뇌피질성형효질세포수종급수통도단백-4(AQP4)표체적영향.방법 원대배양SD대서뇌피질성형효질세포.분별용록화안(5 mmol/L)공배양세포6、12、24、48 h;이갑아풍급이병분(10 μmol/L)예처리세포30 min후여록화안공배양24 h,용단백면역인적법(Western blot)검측성형효질세포AQP4단백표체,광학현미경관찰세포형태,3-4,5-이갑기새서-2,5-이분사서추화물(MTT)검측각조세포활성.결과 록화안처리성형효질세포12 h후AQP4표체개시상조,일직지속도48 h,기중24 h시AQP4표체상조최위명현,이병분예처리조AQP4표체교NH4Cl-24 h조명현강저(P<0.05);광학현미경관찰발현NH4Cl-24 h조세포교정상조화이병분예처리조세포종창명현;MTT검측현시이병분예처리조세포활성교NH4Cl-24 h조화이갑아풍조명현증고(P<0.05).결론 이병분예처리능억제록화안인기적대서뇌피질성형효질세포AQP4표체적상조,감경세포종창정도,제고성형효질세포활성.
Objective To investigate the effect of propofol upon ammonia-induced neocortical astrocyte swelling and aquaporin-4 expression in rat astrocyte. Methods Methods Astrocytes were isolated from newbern Sprague Dawley rats. After a 3-week culture, cell immunofluorescence was employed to label glial fibrillary acidic protein, the specific protein of astrocyte. Astrocytes were cultured with NH4Cl(5mmol/ L) for 6, 12, 24 and 48 h. Astrocytes were pretreated by propofol and DMSO respectively for 30 min and then exposed to NH4Cl for 24h. The expression of AQP4 was detected by the Western blot; cell morphology assessed by light microscopy and cell viability measured by MTT reduction assay. Results The expression of AQP4 was elevated after a 12h exposure to ammonia, lasted to 48h and peaked at 24h. Astrocytes were found significantly swelting in the NH4Cl-24h and DMSO pretreated groups as compared with the propofol pretreated group. The over-expression of aquaporin-4 was attenuated by propofol pretreatment and the cell viability of astrocytes in the propofol pretreated group was higher than that in the NH4Cl-24h group (P< 0.05). Conclusion Pretreatment of astrocytes with propofol can inhibit the over-expression of AQP4, relieve cellular swelling and reduce the ammonia-induced decline in cell viability of astrocytes.
