肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2012年
4期
229-231,242
,共4页
乳腺肿瘤%抗药性,肿瘤%多药耐药相关蛋白质类
乳腺腫瘤%抗藥性,腫瘤%多藥耐藥相關蛋白質類
유선종류%항약성,종류%다약내약상관단백질류
Breast neoplasms%Drug resistance,neoplasm%Multidrug resistance-associated proteins
目的 比较多药耐药相关蛋白1( MRP1)在人类乳腺癌敏感细胞(MCF-7/S)和耐药细胞( MCF-7/TAM、MCF-7/ADR)中的表达差异.初步探索乳腺癌细胞对三苯氧胺(TAM)和多柔比星(ADR)的耐药性的发生机制.方法 分别采用实时荧光定量PCR、Western blot技术检测MRP1基因和MRP1蛋白在3种乳腺癌细胞中的表达差异.结果 MRP1 mRNA在MCF-7/TAM和MCF-7/ADR细胞中表达量分别是MCF-7/S细胞的(2.63 ±0.18)倍和(8.38±0.76)倍,差异均有统计学意义(P=0.004,P=0.003).MRP1蛋白在MCF-7/TAM细胞和MCF-7/ADR细胞中表达均高于MCF-7/S细胞,与MRP1 mRNA的趋势相一致.结论 MRP1的高表达可能是乳腺癌细胞对TAM和ADR产生耐药的共同发生机制.
目的 比較多藥耐藥相關蛋白1( MRP1)在人類乳腺癌敏感細胞(MCF-7/S)和耐藥細胞( MCF-7/TAM、MCF-7/ADR)中的錶達差異.初步探索乳腺癌細胞對三苯氧胺(TAM)和多柔比星(ADR)的耐藥性的髮生機製.方法 分彆採用實時熒光定量PCR、Western blot技術檢測MRP1基因和MRP1蛋白在3種乳腺癌細胞中的錶達差異.結果 MRP1 mRNA在MCF-7/TAM和MCF-7/ADR細胞中錶達量分彆是MCF-7/S細胞的(2.63 ±0.18)倍和(8.38±0.76)倍,差異均有統計學意義(P=0.004,P=0.003).MRP1蛋白在MCF-7/TAM細胞和MCF-7/ADR細胞中錶達均高于MCF-7/S細胞,與MRP1 mRNA的趨勢相一緻.結論 MRP1的高錶達可能是乳腺癌細胞對TAM和ADR產生耐藥的共同髮生機製.
목적 비교다약내약상관단백1( MRP1)재인류유선암민감세포(MCF-7/S)화내약세포( MCF-7/TAM、MCF-7/ADR)중적표체차이.초보탐색유선암세포대삼분양알(TAM)화다유비성(ADR)적내약성적발생궤제.방법 분별채용실시형광정량PCR、Western blot기술검측MRP1기인화MRP1단백재3충유선암세포중적표체차이.결과 MRP1 mRNA재MCF-7/TAM화MCF-7/ADR세포중표체량분별시MCF-7/S세포적(2.63 ±0.18)배화(8.38±0.76)배,차이균유통계학의의(P=0.004,P=0.003).MRP1단백재MCF-7/TAM세포화MCF-7/ADR세포중표체균고우MCF-7/S세포,여MRP1 mRNA적추세상일치.결론 MRP1적고표체가능시유선암세포대TAM화ADR산생내약적공동발생궤제.
Objective To compare the differential expression of MRP1 in human breast cancer sensitive cells (MCF-7/S)and resistant cells (MCF-7/TAM、MCF-7/ADR), and to preliminary distinguish whether there is a common mechanism between breast cancer cells resistant to tamoxifen (TAM) and to adriamycin(ADR).Methods We detected the expression of MRP1 gene and MRP1 protein in the three breast cancer cells by using the methods of real-time fluorescence quantitative PCR, and Western blot respectively.Results The expression of MRP1 gene in MCF-7/TAM and MCF-7/ADR cells was (2.63 ± 0.18) and (8.38 ±0.76) times of MCF-7/S cells respectively, which was significantly different (P1=0.004,P2=0.003). The expression of MRP1 protein in MCF-7/TAM and MCF-7/ADR cells was higher than MCF-7/S cells,which was consistent with the trend of MRP1 gene. Conclusions The high expression of MRP1 may be the common mechanism in breast cancer cells resistant to TAM and ADR.
