中国兽医杂志
中國獸醫雜誌
중국수의잡지
CHINESE JOURNAL OF VETERINARY MEDICINE
2001年
4期
3-5
,共3页
梁华丽%顾亚仙%徐辉%华炯钢
樑華麗%顧亞仙%徐輝%華炯鋼
량화려%고아선%서휘%화형강
HI抗体%鸡新城疫%非典型鸡新城疫%病毒
HI抗體%鷄新城疫%非典型鷄新城疫%病毒
HI항체%계신성역%비전형계신성역%병독
应用血清学、生物学方法,从我省产蛋量下降、HI抗体在1∶128以上的疑似非典型鸡新城疫(CND)病鸡群中分离到1株病毒,经鉴定和致病力测定,明确是1株ND强毒,毒力与F48E9相近;然后采用该株野毒感染不同抗体水平的3批蛋鸡及肉鸡,结果3批蛋鸡均是低抗体滴度组(1∶10~1∶40)发生了CND症状,感染后6~7天开始产蛋停止,经15~17天后才恢复产蛋,高抗体滴定组(1∶80以上)无明显变化,无抗体对照组出现典型鸡新城疫变化,全部死亡;病毒感染后第6、10天监测HI抗体,结果低滴度组第6天达1∶80~1∶160,第10天达1∶160~1∶1280。试验结果表明,我们分离到的NDV,既可引起典型的ND,也可引起非典型的ND;高HI抗体蛋鸡发生非典型鸡新城疫的病因之一,是由于存在低抗体鸡群而发生了CND,其高HI抗体有部分是由于强毒感染后产生的抗体。
應用血清學、生物學方法,從我省產蛋量下降、HI抗體在1∶128以上的疑似非典型鷄新城疫(CND)病鷄群中分離到1株病毒,經鑒定和緻病力測定,明確是1株ND彊毒,毒力與F48E9相近;然後採用該株野毒感染不同抗體水平的3批蛋鷄及肉鷄,結果3批蛋鷄均是低抗體滴度組(1∶10~1∶40)髮生瞭CND癥狀,感染後6~7天開始產蛋停止,經15~17天後纔恢複產蛋,高抗體滴定組(1∶80以上)無明顯變化,無抗體對照組齣現典型鷄新城疫變化,全部死亡;病毒感染後第6、10天鑑測HI抗體,結果低滴度組第6天達1∶80~1∶160,第10天達1∶160~1∶1280。試驗結果錶明,我們分離到的NDV,既可引起典型的ND,也可引起非典型的ND;高HI抗體蛋鷄髮生非典型鷄新城疫的病因之一,是由于存在低抗體鷄群而髮生瞭CND,其高HI抗體有部分是由于彊毒感染後產生的抗體。
응용혈청학、생물학방법,종아성산단량하강、HI항체재1∶128이상적의사비전형계신성역(CND)병계군중분리도1주병독,경감정화치병력측정,명학시1주ND강독,독력여F48E9상근;연후채용해주야독감염불동항체수평적3비단계급육계,결과3비단계균시저항체적도조(1∶10~1∶40)발생료CND증상,감염후6~7천개시산단정지,경15~17천후재회복산단,고항체적정조(1∶80이상)무명현변화,무항체대조조출현전형계신성역변화,전부사망;병독감염후제6、10천감측HI항체,결과저적도조제6천체1∶80~1∶160,제10천체1∶160~1∶1280。시험결과표명,아문분리도적NDV,기가인기전형적ND,야가인기비전형적ND;고HI항체단계발생비전형계신성역적병인지일,시유우존재저항체계군이발생료CND,기고HI항체유부분시유우강독감염후산생적항체。
With the application of serology and biology, A strain of Newcastle disease (ND) virus was isolated from a layer chicken flock with non-typical ND symptoms. The ND HI titers of the layer flock were above 1∶128. The consequence of judgment and test of MDT, ICPI and IVPI showed that the NDV isolated was virulent virus. Also its virulence was nearly the same as F48E9. Three layer groups with the different ND antibody levels were infected with a field isolate of NDV experimentally.The consequence showed that three layer groups with low levels of ND HI antibody (1∶10~1∶40) showed CND symptoms and the egg production stopped at 6th (or 7th) day, the egg production restarted at 15th (or 16th or 17th) day.The group with high levels of ND HI antibody (1∶80 or above) didn't show any clinical symptoms.The group of non-vaccinated chicken showed typical ND clinical symptoms,all of them died of infection. HI antibody of the group with low levels of ND HI antibody reached 1∶80~1∶160 at 6th day,reached 1∶160~1∶1280 at 10th day.The consequence showed that the NDV isolated may cause either typical ND or non-typical ND.One cause of the layer chickens with high levels of ND HI antibody being infected was that the group was infected with CND when its ND HI antibody level was low, part of the high level HI antibody was produced with infection of virulent virus.