CAJ | 학술논문

目的研究粘萎陵菜含有的一种化合物,d-儿茶素-3-O-β-D-葡萄糖苷(CGS)的保肝作用机制.方法测定CGS在体外对NADPH-维生素C和Fe2+-半胱氨酸系统诱发的微粒体脂质过氧化反应(丙二醛形成)的影响,对黄嘌呤-黄嘌呤氧化酶系统超氧阴离子自由基产生(还原型细胞色素C形成)的影响;在体内对CCl4和乙醇诱发的小鼠肝丙二醛形成的影响.结果体外CGS 100 μmol·L-1能明显抑制NADPH-维生素C和Fe2+- 半胱氨酸系统诱发的大鼠脑、肝、肾微粒体丙二醛形成及黄嘌呤-黄嘌呤氧化酶系统超氧阴离子自由基的产生.在体内能抑制CCl4和乙醇诱发的小鼠肝脂质丙二醛形成.结论 CGS具有抗氧化作用.
목적연구점위릉채함유적일충화합물,d-인다소-3-O-β-D-포도당감(CGS)적보간작용궤제.방법측정CGS재체외대NADPH-유생소C화Fe2+-반광안산계통유발적미립체지질과양화반응(병이철형성)적영향,대황표령-황표령양화매계통초양음리자자유기산생(환원형세포색소C형성)적영향;재체내대CCl4화을순유발적소서간병이철형성적영향.결과체외CGS 100 μmol·L-1능명현억제NADPH-유생소C화Fe2+- 반광안산계통유발적대서뇌、간、신미립체병이철형성급황표령-황표령양화매계통초양음리자자유기적산생.재체내능억제CCl4화을순유발적소서간지질병이철형성.결론 CGS구유항양화작용.
AIM To investigate the mechanism of d-catechin-3-O-β-D-glycoside(CGS), one of the compounds from the root of Potentilla viscosa, on liver protection. METHODS The effects of CGS on the lipid peroxidation[malondialdehyde(MDA) formation] in microsomes of rat brain, liver and kidney induced by NADPH-vitamin C and ferrous-cysteine systems in vitro, the superoxide anion radical production(reduced cytochrome C formation) in xanthine-xanthine oxidase system in vitro, and CCl4- and ethanol-induced MDA formation in mouse liver in vivo were evaluated. RESULTS CGS 100 μmol·L-1 inhibited MDA formation and the superoxide anion radical production in microsomes of rat brain, liver and kidney in vitro, and MDA formation in mouse liver in vivo. CONCLUSION CGS exhibits an antioxidant activity.