CAJ | 학술논문

以籼粳交"密阳23/吉冷1号"的F2:3代200个家系作为作图群体,在12℃冷水胁迫下,进行苗高、苗鲜重和苗干重等水稻幼苗活力性状的低温反应鉴定,并利用由SSR标记构建的分子连锁图谱为基础,对冷水胁迫下苗高、苗鲜重和苗干重以及它们的低温反应指数进行了数量性状基因座(QTLs)检测.研究结果表明,低温胁迫下上述幼苗活力性状在F3家系群中均表现为接近正态的连续分布,表现为由多基因控制的数量性状;在第1、2、7、8和12染色体上,检测到与幼苗活力性状的低温反应相关的QTL共12个,对表型变异的贡献率范围为5.2%～17.9%,其中位于第2染色体RM262-RM263区间和第12染色体RM270-RM17区间的与低温下苗高相关的qCSH2和qCSH12,以及位于第12染色体RM19-RM270区间和第1染色体RM129-RM9区间的分别控制低温下苗干重及其低温反应指数的qSDW12和qCSDW1对表型变异的贡献率较大,分别为16.6%、17.9%、15.9%和16.2%.其增效等位基因均来自吉冷1号,前两者均表现为加性效应,后两者分别表现为显性和超显性.
이선갱교"밀양23/길랭1호"적F2:3대200개가계작위작도군체,재12℃랭수협박하,진행묘고、묘선중화묘간중등수도유묘활력성상적저온반응감정,병이용유SSR표기구건적분자련쇄도보위기출,대랭수협박하묘고、묘선중화묘간중이급타문적저온반응지수진행료수량성상기인좌(QTLs)검측.연구결과표명,저온협박하상술유묘활력성상재F3가계군중균표현위접근정태적련속분포,표현위유다기인공제적수량성상;재제1、2、7、8화12염색체상,검측도여유묘활력성상적저온반응상관적QTL공12개,대표형변이적공헌솔범위위5.2%～17.9%,기중위우제2염색체RM262-RM263구간화제12염색체RM270-RM17구간적여저온하묘고상관적qCSH2화qCSH12,이급위우제12염색체RM19-RM270구간화제1염색체RM129-RM9구간적분별공제저온하묘간중급기저온반응지수적qSDW12화qCSDW1대표형변이적공헌솔교대,분별위16.6%、17.9%、15.9%화16.2%.기증효등위기인균래자길랭1호,전량자균표현위가성효응,후량자분별표현위현성화초현성.
The quantitative trait loci (QTLs) for the seedling vigor traits under 12℃ cold water irrigation, such as the seedling height, the seedling fresh weight, the seedling dry weight, and their cold response index, were identified using an F2:3 population including 200 individuals and lines derived from a cross of indica and japonica "Milyang 23/Jileng 1" with microsatellite markers.All seedling vigor traits exhibited a continuous distribution near normal in F3 lines; these traits were quantitative traits controlled by multiple genes. Twelve QTLs confemng the seedling vigor traits under cold water irrigation were detected on chromosomes 1, 2, 7,8, and 12, which explained the observed phenotypic variance from 5.2% to 17.9%. Among them, qCSH2 and qCSH12 were located in RM262-RM263 on chromosome 2 and RM270-RM17 on chromosome 12, respectively, which were associated with the seedling height. qSDW12 and qCSDW1 were located in RM19-RM270 on chromosome 12 and RM129-RM9 on chromosome 1, respectively, which were correlated with the seedling dry weight and its cold response index, and the explained 16.6%, 17.9%, 15.9%, and 16.2% of the observed phenotypic variation, respectively. These QTLs alleles were derived from cold-tolerant parent Jileng 1; the gene actions of the two front genes showed their additive effect, and the two genes belind showed dominant and over dominant effects, respectively.