CAJ | 학술논문

目的:通过重组cDNA文库的血清学筛选(SEREX)技术获得来自非洲爪蟾(Xenopus laevis)肝脏肿瘤文库中具有潜在治疗价值的异种同源抗原的cDNA序列.方法:用人肝癌细胞株SMMC-7721免疫家兔后收集的抗血清作为筛选血清,采用SEREX技术筛选Xenopus laevis肝脏肿瘤cDNA表达文库.结果:通过两轮筛选获得19个阳性克隆,通过DNA序列测定和分子生物学技术确定这19个克隆编码16个蛋白.其中Transgelin 2、RAS guanyl releasing protein 1和Chromobox protein homolog 6可能与肝细胞肝癌有关.结论:用异种免疫血清筛选异种同源肿瘤抗原拓宽了SEREX 技术的应用范围,鉴定Transgelin 2、RAS guanyl releasing protein 1和Chromobox protein homolog 6可能为异种同源抗原.
목적:통과중조cDNA문고적혈청학사선(SEREX)기술획득래자비주조섬(Xenopus laevis)간장종류문고중구유잠재치료개치적이충동원항원적cDNA서렬.방법:용인간암세포주SMMC-7721면역가토후수집적항혈청작위사선혈청,채용SEREX기술사선Xenopus laevis간장종류cDNA표체문고.결과:통과량륜사선획득19개양성극륭,통과DNA서렬측정화분자생물학기술학정저19개극륭편마16개단백.기중Transgelin 2、RAS guanyl releasing protein 1화Chromobox protein homolog 6가능여간세포간암유관.결론:용이충면역혈청사선이충동원종류항원탁관료SEREX 기술적응용범위,감정Transgelin 2、RAS guanyl releasing protein 1화Chromobox protein homolog 6가능위이충동원항원.