检验医学
檢驗醫學
검험의학
LABORATORY MEDICINE
2009年
7期
493-496
,共4页
Rh血型系统%RhD基因%多态性%聚合酶链反应%多重
Rh血型繫統%RhD基因%多態性%聚閤酶鏈反應%多重
Rh혈형계통%RhD기인%다태성%취합매련반응%다중
Rh blood group system%RhD gene%Polymorphism%Polymerase chain reaction,multiplex
目的 研究山东汉族人群RhD阴性个体RhD基因多态性.方法 采用标准血清学方法和抗球蛋白实验筛选Rh阴性个体;对Rh阴性个体再用吸收放散实验确定DEL型.运用多重聚合酶链反应(PCR)方法分析RhD阴性个体基因存在情况.结果 67例Rh阴性个体中DEL型16例,占Rh阴性个体的23.88%,其6个RhD基因特异性的第3、4、5、6、7、9外显子全部存在;剩下51例Rh阴性个体中1例缺失第5外显子,其余50例6个外显子全部缺失.结论 山东汉族人群RhD阴性个体中存在一定比例的DEL型,且所有DEL样本均具有完整的RhD基因,在排除DEL型后的RhD阴性个体可能携带RhD基因,但其比例较低.
目的 研究山東漢族人群RhD陰性箇體RhD基因多態性.方法 採用標準血清學方法和抗毬蛋白實驗篩選Rh陰性箇體;對Rh陰性箇體再用吸收放散實驗確定DEL型.運用多重聚閤酶鏈反應(PCR)方法分析RhD陰性箇體基因存在情況.結果 67例Rh陰性箇體中DEL型16例,佔Rh陰性箇體的23.88%,其6箇RhD基因特異性的第3、4、5、6、7、9外顯子全部存在;剩下51例Rh陰性箇體中1例缺失第5外顯子,其餘50例6箇外顯子全部缺失.結論 山東漢族人群RhD陰性箇體中存在一定比例的DEL型,且所有DEL樣本均具有完整的RhD基因,在排除DEL型後的RhD陰性箇體可能攜帶RhD基因,但其比例較低.
목적 연구산동한족인군RhD음성개체RhD기인다태성.방법 채용표준혈청학방법화항구단백실험사선Rh음성개체;대Rh음성개체재용흡수방산실험학정DEL형.운용다중취합매련반응(PCR)방법분석RhD음성개체기인존재정황.결과 67례Rh음성개체중DEL형16례,점Rh음성개체적23.88%,기6개RhD기인특이성적제3、4、5、6、7、9외현자전부존재;잉하51례Rh음성개체중1례결실제5외현자,기여50례6개외현자전부결실.결론 산동한족인군RhD음성개체중존재일정비례적DEL형,차소유DEL양본균구유완정적RhD기인,재배제DEL형후적RhD음성개체가능휴대RhD기인,단기비례교저.
Objective To study the RhD gene polymorphism among RhD-negative Han population in Shandong province. Methods The RhD-negative individuals were screened by the standard serological method and antiglobulin test;The DEL individuals were identified by absorption/elution method. RhD gene polymorphisms in RhD-negative individuals were analyzed by multiplex polymerase chain reaction (PCR). Results 16 of 17 individuals were identified as DEL and the percentage of DEL was 23.88%.All DEL individuals had all the six RhD-specific exons (Exon 3, 4, 5, 6, 7 and 9). The RhD-negative individuals showed two polymorphisms: 1 of 51 individuals lacked the fifth exon and the others of them lacked all the six RhD-specific exons. Conclusions DEL phenotype is highly detected among RhD-negative Han population in Shandong province. DEL individuals have intact RhD gene .The RhD-negative individuals excluding DEL phenotyping may carry RhD gene. However, the carrying proportion is low.
