食品科学
食品科學
식품과학
FOOD SCIENCE
2009年
22期
293-297
,共5页
张弛%楮庆华%孟瑾%杨捷琳%顾鸣%韩奕奕%沈鹤柏%赵渝%郭鲁申%李敏%支援%包建强
張弛%楮慶華%孟瑾%楊捷琳%顧鳴%韓奕奕%瀋鶴柏%趙渝%郭魯申%李敏%支援%包建彊
장이%저경화%맹근%양첩림%고명%한혁혁%침학백%조투%곽로신%리민%지원%포건강
沙门氏菌%磁性纳米粒子%逆转录聚合酶链式反应(RT-PCR)
沙門氏菌%磁性納米粒子%逆轉錄聚閤酶鏈式反應(RT-PCR)
사문씨균%자성납미입자%역전록취합매련식반응(RT-PCR)
Salmonella%magnetic nanoparticles(MNPs)%reverse transcription-polymerase chain reaction (RT-PCR)
建立适合食源性沙门氏菌的RT-PCR检测体系.根据沙门氏菌的转录起始因子rpoD设计引物Salmrpod2/5,以rpoD基因的mRNA为检测对象,在样品制备时采用新型的磁性纳米粒子分离mRNA技术,建立快速检测食品中沙门氏菌的逆转录聚合酶链式反应(RT-PCR)的方法.结果表明,Salmrpod2/5引物能有效地将沙门氏菌与其他亲缘关系较近的肠杆菌科细菌区分开来.样品中沙门氏菌添加实验表明,该方法对沙门氏菌的检测下限为10CFU/25ml,检测时间少于18h.该方法具有灵敏、特异、快速的特点,适宜于在食品卫生行业中进行推广及应用.
建立適閤食源性沙門氏菌的RT-PCR檢測體繫.根據沙門氏菌的轉錄起始因子rpoD設計引物Salmrpod2/5,以rpoD基因的mRNA為檢測對象,在樣品製備時採用新型的磁性納米粒子分離mRNA技術,建立快速檢測食品中沙門氏菌的逆轉錄聚閤酶鏈式反應(RT-PCR)的方法.結果錶明,Salmrpod2/5引物能有效地將沙門氏菌與其他親緣關繫較近的腸桿菌科細菌區分開來.樣品中沙門氏菌添加實驗錶明,該方法對沙門氏菌的檢測下限為10CFU/25ml,檢測時間少于18h.該方法具有靈敏、特異、快速的特點,適宜于在食品衛生行業中進行推廣及應用.
건립괄합식원성사문씨균적RT-PCR검측체계.근거사문씨균적전록기시인자rpoD설계인물Salmrpod2/5,이rpoD기인적mRNA위검측대상,재양품제비시채용신형적자성납미입자분리mRNA기술,건립쾌속검측식품중사문씨균적역전록취합매련식반응(RT-PCR)적방법.결과표명,Salmrpod2/5인물능유효지장사문씨균여기타친연관계교근적장간균과세균구분개래.양품중사문씨균첨가실험표명,해방법대사문씨균적검측하한위10CFU/25ml,검측시간소우18h.해방법구유령민、특이、쾌속적특점,괄의우재식품위생행업중진행추엄급응용.
The main object of this work was to establish a RT-PCR system suitable for Salmonella detection in foodstuffs. A primer Salmrpod2/5 based on the major sigma factor of Salmonella Methods was designed and mRNA in rpoD gene was analyzed. mRNA from bacteria was isolated by magnetic nanoparticles technique. A sensitive, time saving and specific method of reverse transcription-polymerase chain reaction (RT-PCR) combined with magnetic nanoparticle was developed to detect Salmonella. The results indicated amplification on the site of 260bp in Salmonella-specific gene fragment using the primer Salmrpod2/5. Signals from Salmonella were obtained and no cross-reaction was observed with other members of the Enterobacteriaceae. The detection limit of RT-PCR method was less than 10 CFU in 25 ml samples. The whole analysis took less than 18 h. In summary, this method shows advantages with highly specific, sensitive and quick in detection. It would be very useful for the rapid detection of Salmonella spp. in the food and the health industry.
