CAJ | 학술논문

通过SDS-PAGE电泳对牛血清中纤维蛋白原检测方法进行研究,确定检测参数为分离胶浓度12.5%、交联度3%、浓缩胶浓度5%.结果表明,采用4℃温度条件下、21%饱和硫酸铵溶液对牛血清中的纤维蛋白原进行分离,所得血清中纤维蛋白原的残留量仪为0.02473%.
통과SDS-PAGE전영대우혈청중섬유단백원검측방법진행연구,학정검측삼수위분리효농도12.5%、교련도3%、농축효농도5%.결과표명,채용4℃온도조건하、21%포화류산안용액대우혈청중적섬유단백원진행분리,소득혈청중섬유단백원적잔류량의위0.02473%.
Fibrinogen in bovine serum was determined using tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method, and the optimum condition were obtained as follows: separation gel concentration 12.5%, cross linking degree 3% and the concentration of spacer gel 5%. Fibrinogen was separated from bovine serum using 21% ammonium sulfate precipitation at 4 ℃, and the fibrinogen residue in bovine serum was reduced to 0.02473%.