CAJ | 학술논문

从水团花乙醇提物的乙酸乙酯部位分离到6个黄酮类化合物,根据光谱数据分别鉴定为柚皮素(1)、圣草酚(2)、槲皮素(3)、柚皮素7-O-β-D-葡萄糖苷(4)、圣草酚-7-O-β-D-葡萄糖苷(5)、槲皮素-3-O-β-D-葡萄糖苷(6),化合物1、2、4和5为首次从该植物中分离得到.采用细胞病变抑制法(CPE reduction assay)和MTT法测定化合物的体外抗病毒活性,结果显示,三个黄酮苷元1、2和3均具有不同程度的体外抑制呼吸道合胞病毒(RSV)和柯萨奇B3型病毒(CVB3)活性,反之,3个黄酮苷均不显示活性.
종수단화을순제물적을산을지부위분리도6개황동류화합물,근거광보수거분별감정위유피소(1)、골초분(2)、곡피소(3)、유피소7-O-β-D-포도당감(4)、골초분-7-O-β-D-포도당감(5)、곡피소-3-O-β-D-포도당감(6),화합물1、2、4화5위수차종해식물중분리득도.채용세포병변억제법(CPE reduction assay)화MTT법측정화합물적체외항병독활성,결과현시,삼개황동감원1、2화3균구유불동정도적체외억제호흡도합포병독(RSV)화가살기B3형병독(CVB3)활성,반지,3개황동감균불현시활성.
Six flavonoids were isolated from the ethyl acetate fraction of the ethanol extract of Adina pilulifera. The chemical structures of these flavonoids were determined by spectroscopic analysis,and identified to be naringenin(l) .eriodic-tyol(2) ,quercetin(3) ,naringenin-7-0-β-O-glucopyranside(4) , eriodictyol-7-O-β-D-glucopyranside(5) ,and quercetin-3-O-β-D-glucopyranside(6). Among them,compounds 1,2,4 and 5 were isolated from A. Pilulifera for the first time. Antiviral tests using cytopathic effect(CPE) reduction assay and MTT method revealed that the flavonoid aglycones 1,2 and 3 possess in vitro antiviral activity against both respiratory syncytial virus (RSV) and coxsackie B3 virus ( CVB3) to different extents. In contrast,the flavonoid glucosides 4,5 and 6 did not show any anti-RSV and anti-CVB3 effects.