CAJ | 학술논문

[目的]探讨多胺增强植物抗旱性的机理,为多胺作为外源牛长调节物质在玉米抗旱过程中的应用提供理论基础.[方法]以聚乙二醇(PEG-6000)模拟自然干旱,用外源Spa处理抗旱性不同的2个玉米品种农大108和掖单13,检测渗透胁迫下2个品种幼苗叶片中相对含水量及可溶性蛋白含量的变化.可溶性蛋白含量的测定采用考马斯亮蓝法.[结果]渗透胁迫7 d,抗旱性弱的掖单13玉米幼苗叶片的相对含水量和可溶性蛋白含量的下降幅度明显大于抗旱性较强的品种农大108;外源Spd处理明显抑制掖单13在胁迫条件下其幼苗叶片相对含水量的下降,并且提高了其在胁迫条件下可溶性蛋白的含量.可能是因为外源Spd处理,促进其合成了一些特异蛋白,在胁迫条件下发挥其特异功能,以确保水分胁迫下各类物质代谢的适应性变化,增强自身的抗旱性.[结论]外源Spd处理,通过提高渗透胁迫下玉米幼苗叶片内蛋白质的含量,提高了玉米幼苗的抗渗透胁迫能力.
[목적]탐토다알증강식물항한성적궤리,위다알작위외원우장조절물질재옥미항한과정중적응용제공이론기출.[방법]이취을이순(PEG-6000)모의자연간한,용외원Spa처리항한성불동적2개옥미품충농대108화액단13,검측삼투협박하2개품충유묘협편중상대함수량급가용성단백함량적변화.가용성단백함량적측정채용고마사량람법.[결과]삼투협박7 d,항한성약적액단13옥미유묘협편적상대함수량화가용성단백함량적하강폭도명현대우항한성교강적품충농대108;외원Spd처리명현억제액단13재협박조건하기유묘협편상대함수량적하강,병차제고료기재협박조건하가용성단백적함량.가능시인위외원Spd처리,촉진기합성료일사특이단백,재협박조건하발휘기특이공능,이학보수분협박하각류물질대사적괄응성변화,증강자신적항한성.[결론]외원Spd처리,통과제고삼투협박하옥미유묘협편내단백질적함량,제고료옥미유묘적항삼투협박능력.
[Objective]The research aimed to probe into the mechanism of polyamine enhancing the tolerance of plants to drought stress and provide theoretical basis for application of polyamine in process of maize drought resistance.[Method]With PEG-6000 simulating natural drought, the change in content of soluble protein and relative water content were investigated in seedling leaves of two maize cultivars, Nongda 108 and Yedan 13 under osmotic stress with exogenous Spd treatment.[Result]On the 7th day, leaf relative water content and the content of soluble protein decreased more significantly in leaves of Yedan 13(drought-sensitive)than in Nongda 108(drought-tolerant).Exogenous Spd treatment not only obviously inhibited the decrease of leaf relative water content, but also increased the content of soluble protein.[Conclusion]Exogenous Spd treatment could enhance the tolerance of maize seedlings to osmotic stress, via improving the content of soluble protein in seedling leaves.