中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2009年
1期
37-41
,共5页
江洁华%谭获%徐军%廖伟娇%易健云%张还珠%李翊泉%郑贵星%许志晟%朱伯平
江潔華%譚穫%徐軍%廖偉嬌%易健雲%張還珠%李翊泉%鄭貴星%許誌晟%硃伯平
강길화%담획%서군%료위교%역건운%장환주%리익천%정귀성%허지성%주백평
革兰阴性茵/致病力%基因%抗药性,微生物%聚合酶链反应
革蘭陰性茵/緻病力%基因%抗藥性,微生物%聚閤酶鏈反應
혁란음성인/치병력%기인%항약성,미생물%취합매련반응
Gram-negative bacteria/PY%Genes%Drug resistance,microbial%Polymorphism chain reaction
目的 探讨强致病岛(HPI)fyuA-irp2基因簇在多重耐药革兰阴性杆菌中的分布及分子生物学特征.方法 用多重聚合酶链式反应(PCR)技术扩增84株茵的加fyuA-irp2基因簇,时产物进行DNA测序.结果 irp1、irp2、irp3、irp4及fyuA的总检出率分别是:40.48%、41.67%、5.95%、0%及16.67%;EC06748、Kp7151及PAE 7之fyuA蛋白质序列与YP_853080的相比有100%同源;克雷伯菌49及Kp51之irp2与AAA27636.1的同源性高(99%),而大肠埃希茵ECO4、EC07与1176840的相符率低(90%).GenBank登陆号分别为:FJ211852及FJ211851;Kp51、Kp10及Kp49之irp1均与AL590842有99%同源;EC03、Kp51、Kp10及Kp49之irp3与CAA73128相比有97%的同源.同种茵株突变的位点大致相同,不同种菌株间的突变差异较大.结论 在多重耐药革兰阴性杆茵中检出HPI,fyuA-irp2基因簇有不同程度的突变和缺失.
目的 探討彊緻病島(HPI)fyuA-irp2基因簇在多重耐藥革蘭陰性桿菌中的分佈及分子生物學特徵.方法 用多重聚閤酶鏈式反應(PCR)技術擴增84株茵的加fyuA-irp2基因簇,時產物進行DNA測序.結果 irp1、irp2、irp3、irp4及fyuA的總檢齣率分彆是:40.48%、41.67%、5.95%、0%及16.67%;EC06748、Kp7151及PAE 7之fyuA蛋白質序列與YP_853080的相比有100%同源;剋雷伯菌49及Kp51之irp2與AAA27636.1的同源性高(99%),而大腸埃希茵ECO4、EC07與1176840的相符率低(90%).GenBank登陸號分彆為:FJ211852及FJ211851;Kp51、Kp10及Kp49之irp1均與AL590842有99%同源;EC03、Kp51、Kp10及Kp49之irp3與CAA73128相比有97%的同源.同種茵株突變的位點大緻相同,不同種菌株間的突變差異較大.結論 在多重耐藥革蘭陰性桿茵中檢齣HPI,fyuA-irp2基因簇有不同程度的突變和缺失.
목적 탐토강치병도(HPI)fyuA-irp2기인족재다중내약혁란음성간균중적분포급분자생물학특정.방법 용다중취합매련식반응(PCR)기술확증84주인적가fyuA-irp2기인족,시산물진행DNA측서.결과 irp1、irp2、irp3、irp4급fyuA적총검출솔분별시:40.48%、41.67%、5.95%、0%급16.67%;EC06748、Kp7151급PAE 7지fyuA단백질서렬여YP_853080적상비유100%동원;극뢰백균49급Kp51지irp2여AAA27636.1적동원성고(99%),이대장애희인ECO4、EC07여1176840적상부솔저(90%).GenBank등륙호분별위:FJ211852급FJ211851;Kp51、Kp10급Kp49지irp1균여AL590842유99%동원;EC03、Kp51、Kp10급Kp49지irp3여CAA73128상비유97%적동원.동충인주돌변적위점대치상동,불동충균주간적돌변차이교대.결론 재다중내약혁란음성간인중검출HPI,fyuA-irp2기인족유불동정도적돌변화결실.
Objective To investigate the distribution of high pathogenicity island(HPI)in multiple-drug-resistance gram-negative bacilli and analyze the protein sequence.Methods To amplify thefyuA-irp2 gene cluster of the 84 isolates by multiple polymerase chain reaction(PCR),the product was subsequently sequenced.Results The positive rate ofirpl,irp2,irp3,irp4 and fyuA was 40.48％,41,67％,5.95％,O％and 16.67％,respectively.Theamino sequence offyuA comefromEC06748,Kp7151 and PAE7 was usedto compare with AL590842,there are 100％identities.Amino sequence ofirp2 come from Kp49 and Kp51 have 99％identities with AAA27636.1,but amino sequence of irp2 come from EC04 and EC07 only have 90％identities with 1176840.The GenBank accession number is FJ211852 and FJ211851.Amino sequence ofirpl come fromKp 10,Kp49 and Kp51 have 99％identities with AL590842。and amino sequence ofirp3 come from EC03,Kp51,Kp10 and Kp49 have 97％identities with CAA73128.There are the same mutation among the same species,and different mutation among different species.Conclusion There was different extant mutant lost in thefy～t-i,v2 gene cluster in multiple-drug-resistanee gram-negative bacilli.
