CAJ | 학술논문

目的根据parC基因序列差异建立一种解脲支原体基因分型方法,以实现其两个生物型Uu(Ureaplasma urealyticum)和Up(Ureaplasma parvum)的快速鉴定,便于临床常规应用.方法依据解脲支原体14个血清型标准株parC基因序列,设计可区分Uu和Up的特异性引物与和探针；通过检测serovarl和serovat4标准株、50份解脲支原体临床分离株(Uu 12株,Up38株),7种阴道常见分离菌以鉴定该方法的特异性；留取70例性病门诊非淋球菌性泌尿生殖道炎症患者和71例妇科正常体检者的标本,分别进行解脲支原体培养和基因分型鉴定,以比较两种方法的敏感性,并应用统计学分析Uu、Up在性病和正常人群中分布的差异.结果应用parC基因分型法成功将serovar1标准株、serovat4标准株、Uu和Up临床分离株鉴别,Uu、Up两生物群之间以及7种阴道常见菌均未出现非特异性扩增；基因分型方法的敏感性显著高于培养法(P＜0.05)；在70例性病门诊患者中,Uu和Up的检出率分别为8.57％和61.40％,两者混合感染为24.30％；在71例妇科体检人群中Uu和Up检出率分别为7.04％和67.60％,两者混合感染为8.45％,性病门诊病人中解脲支原体感染率显著高于体检人群(P＜0.05),Uu和Up单纯感染在两群体中的分布差异无统计学意义(P＞0.05),而混合感染在性病门诊病人中显著增加(P＜0.05).结论解脲支原体在性病患者中的感染率显著高于健康体检者,且混合感染在性病患者中明显增加,而Uu和Up单纯感染在两人群中分布差异无统计学意义,因此解脲支原体的致病可能与其不同基因型的混合感染有关.
목적 근거parC기인서렬차이건립일충해뇨지원체기인분형방법,이실현기량개생물형Uu(Ureaplasma urealyticum)화Up(Ureaplasma parvum)적쾌속감정,편우림상상규응용.방법 의거해뇨지원체14개혈청형표준주parC기인서렬,설계가구분Uu화Up적특이성인물여화탐침；통과검측serovarl화serovat4표준주、50빈해뇨지원체림상분리주(Uu 12주,Up38주),7충음도상견분리균이감정해방법적특이성；류취70례성병문진비림구균성비뇨생식도염증환자화71례부과정상체검자적표본,분별진행해뇨지원체배양화기인분형감정,이비교량충방법적민감성,병응용통계학분석Uu、Up재성병화정상인군중분포적차이.결과 응용parC기인분형법성공장serovar1표준주、serovat4표준주、Uu화Up림상분리주감별,Uu、Up량생물군지간이급7충음도상견균균미출현비특이성확증；기인분형방법적민감성현저고우배양법(P＜0.05)；재70례성병문진환자중,Uu화Up적검출솔분별위8.57％화61.40％,량자혼합감염위24.30％；재71례부과체검인군중Uu화Up검출솔분별위7.04％화67.60％,량자혼합감염위8.45％,성병문진병인중해뇨지원체감염솔현저고우체검인군(P＜0.05),Uu화Up단순감염재량군체중적분포차이무통계학의의(P＞0.05),이혼합감염재성병문진병인중현저증가(P＜0.05).결론 해뇨지원체재성병환자중적감염솔현저고우건강체검자,차혼합감염재성병환자중명현증가,이Uu화Up단순감염재량인군중분포차이무통계학의의,인차해뇨지원체적치병가능여기불동기인형적혼합감염유관.
Objective To establish a novel PCR method that can differentiate the two biotype of Mycoplasma urealytium rapidly based on the disparities of parC gene sequences for clinical routine examination.Methods Design two pairs of specific primers and probes for the targeted gene according to the differences of the parC gene sequences from 14 standard serotypes.The specificity of this amplification was verified by detecting two Mycoplasma urealyticum standard strains including serovar1 and serovar4,50 clinical isolated strains ( 12 are Uu strains and 38 are Up strains) and 7 common bacterias from vagina.Collected 70 swab specimens from patients of Sexually Transmit Department with urogenital inflammation symptom and 71 swab specimens from the gynecology health examination population.All those specimens were detected by culture and our method respectively.The sensitivity of this method was evaluated by comparing with the culture.Differences of the infection rate and distribution of biotypes between different populations were analyzed using statistical software.Results Standard strains of Mycoplasma urealyticum and clinical isolates can be typed into two species by the PCR method without nonspecific amplification.The sensitivity of PCR method is much higher than that of culture ( P＜0.05).The infection rates of Uu,Up and the mixing were 8.57％,61.40％,24.30％ respectively for the patients with vaginitis.However,it was 8.80％,67.60％,8.45％ respectively for the gynecology health examination population.There is a significant difference of the total infection rate between the two population(P＜0.05).It showed no significant difference with the distribution of the two types of simple infection in the two groups ( P＞0.05).But the rate of mixing infection is much higher in the patients with vaginitis ( P＜0.05 ).Conclusion The pathology of Mycoplasma urealyticum may be related to the mixed infection of different biotypes.