中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2008年
4期
422-424
,共3页
安岗%毛伟征%代震波%牛兆建%刘希春%解西河%赵宝成
安崗%毛偉徵%代震波%牛兆建%劉希春%解西河%趙寶成
안강%모위정%대진파%우조건%류희춘%해서하%조보성
胃肿瘤%树突细胞%抗原%治疗
胃腫瘤%樹突細胞%抗原%治療
위종류%수돌세포%항원%치료
Stomach neoplasm%Dendritic cells%Antigen%Therapy
目的 观察抗原致敏树突细胞(DC)与细胞因子诱导的杀伤细胞(CIK)共培养体内外抗胃癌(SCC-7901)的效应.方法 取健康供者外周血体外诱导培养DC和CIK,应用噻唑蓝(MTT)法测定比较(每组n=3)单纯CIK(CIK组)、DC诱导CIK(DC-CIK组)和经胃癌抗原致敏的DC诱导CIK(抗原-DC-CIK组)体外对SGC-7901的杀伤活性,并利用SGC-7901建立胃癌裸鼠模型,设立PBS对照组,比较各组(每组n=5)瘤体体积、抑瘤率和肿瘤坏死面积评分.结果 成熟DC与CIK共培养第7天时,DC-CIK扩增倍数为17.8±2.0,约为单纯CIK扩增倍数(10.9±1.8)的1.63倍(P＜0.05);抗原-DC-CIK组、DC-CIK组、CIK组,体外实验中效靶比为20:1时对SGC-7901的杀伤活性分别为:(72.3±0.5)%、(53.9±0.7)%、(46.4±0.4)%(P均＜0.01);体内动物实验中抑瘤率分别为30.02%、18.11%、15.94%(P均＜0.01).结论 SGC-7901抗原致敏DC能增加CIK的扩增数量,增强CIK对SGC-7901的杀伤作用.
目的 觀察抗原緻敏樹突細胞(DC)與細胞因子誘導的殺傷細胞(CIK)共培養體內外抗胃癌(SCC-7901)的效應.方法 取健康供者外週血體外誘導培養DC和CIK,應用噻唑藍(MTT)法測定比較(每組n=3)單純CIK(CIK組)、DC誘導CIK(DC-CIK組)和經胃癌抗原緻敏的DC誘導CIK(抗原-DC-CIK組)體外對SGC-7901的殺傷活性,併利用SGC-7901建立胃癌裸鼠模型,設立PBS對照組,比較各組(每組n=5)瘤體體積、抑瘤率和腫瘤壞死麵積評分.結果 成熟DC與CIK共培養第7天時,DC-CIK擴增倍數為17.8±2.0,約為單純CIK擴增倍數(10.9±1.8)的1.63倍(P＜0.05);抗原-DC-CIK組、DC-CIK組、CIK組,體外實驗中效靶比為20:1時對SGC-7901的殺傷活性分彆為:(72.3±0.5)%、(53.9±0.7)%、(46.4±0.4)%(P均＜0.01);體內動物實驗中抑瘤率分彆為30.02%、18.11%、15.94%(P均＜0.01).結論 SGC-7901抗原緻敏DC能增加CIK的擴增數量,增彊CIK對SGC-7901的殺傷作用.
목적 관찰항원치민수돌세포(DC)여세포인자유도적살상세포(CIK)공배양체내외항위암(SCC-7901)적효응.방법 취건강공자외주혈체외유도배양DC화CIK,응용새서람(MTT)법측정비교(매조n=3)단순CIK(CIK조)、DC유도CIK(DC-CIK조)화경위암항원치민적DC유도CIK(항원-DC-CIK조)체외대SGC-7901적살상활성,병이용SGC-7901건립위암라서모형,설립PBS대조조,비교각조(매조n=5)류체체적、억류솔화종류배사면적평분.결과 성숙DC여CIK공배양제7천시,DC-CIK확증배수위17.8±2.0,약위단순CIK확증배수(10.9±1.8)적1.63배(P＜0.05);항원-DC-CIK조、DC-CIK조、CIK조,체외실험중효파비위20:1시대SGC-7901적살상활성분별위:(72.3±0.5)%、(53.9±0.7)%、(46.4±0.4)%(P균＜0.01);체내동물실험중억류솔분별위30.02%、18.11%、15.94%(P균＜0.01).결론 SGC-7901항원치민DC능증가CIK적확증수량,증강CIK대SGC-7901적살상작용.
Objective To observe the effect of cytokine induced killer cells(CIK)co-cultured with antigen-pulsed dendritic cells(DC)against gastric cancer(SGC-7901)in vivo and in vitro.Methods DC and CIK were induced and cultivated from peripheral blood of healthy donors.The cytotoxic ability against SGC-7901 in vitro of pure CIK(CIK group),CIK induced by DC(DC-CIK group)and CIK induced by gastric cancer antigen-pulsed DC(Ag-DC-CIK group)was detected and compared by using MTT method:the antitumor activity in vivo of 3 groups above and PBS control group was evaluated in nude mice bearing SGC-7901 cancer by comparing the volume of tumor,the inhibition ratio and the pathologic scores of tumor necrosis area.Results On the 7th day after co-culturing mature CIK with DC,the amplification fold of DC-CIK was 17.8±2.0,which was 1.63 times as many as CIK group(P＜0.05);The cytotoxic ability of Ag-DC-CIK group,DC-CIK group and CIK group against SGC-7901 was(72.3±0.5)%,(53.9±0.7)% and(46.4±0.4)%(P＜0.01),when effector to target ratio was 20:1 in vitro;the inhibition ratio was 30.02%.18.11% and 15.94%(P＜0.01)respectively in vivo.Condusion SGC-7901 antigen-pulsed DC can increase the amplification amount of CIK and enhance the cytotoxic effect of CIK against SGC-7901.
