中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2011年
5期
429-433
,共5页
IL-2%粒细胞集落刺激因子%细胞增殖%细胞极化%混合淋巴细胞反应
IL-2%粒細胞集落刺激因子%細胞增殖%細胞極化%混閤淋巴細胞反應
IL-2%립세포집락자격인자%세포증식%세포겁화%혼합림파세포반응
Interleukin-2%Granulocyte colony-stimulating factor%Cell proliferation%Cell polarization%Mixed lymphocyte reaction
目的 探讨IL-2预孵育对粒细胞集落刺激因子(G-CSF)动员后的小鼠脾淋巴细胞功能的影响,从而为减轻异基因造血干细胞移植时急性移植物抗宿主病(GVHD)的发生提供一种新途径.方法 IL-2(50 U/ml)预孵育rhG-CSF(每天0.25 μg/g)体内动员的C57BL/6N小鼠脾细胞和na(i)ve CD4+T细胞6 h,然后在体外以BALB/c小鼠异基因抗原分别对其进行刺激,测定动员后的C57BL/6N小鼠脾细胞和na(i)ve CD4+T细胞的增殖能力以及上清液IL-4、IFN-γ的浓度,实验分为G-CSF+IL-2组、G-CSF组、IL-2组和对照组.结果 G-CSF+IL-2组较列照组A值降低(P＜0.01);G-CSF+IL-2组IL-4水平高于对照组(P＜0.01),而IFN-γ水平低于对照组(P＜0.01).结论 rhG-CSF动员的C57BL/6N小鼠脾细胞和na(i)ve CD4+T细胞经IL-2预孵育后对同种异基因抗原免疫增殖能力明显减弱并向Th2方向分化,两者合用对诱导T淋巴细胞免疫耐受有协同效应.
目的 探討IL-2預孵育對粒細胞集落刺激因子(G-CSF)動員後的小鼠脾淋巴細胞功能的影響,從而為減輕異基因造血榦細胞移植時急性移植物抗宿主病(GVHD)的髮生提供一種新途徑.方法 IL-2(50 U/ml)預孵育rhG-CSF(每天0.25 μg/g)體內動員的C57BL/6N小鼠脾細胞和na(i)ve CD4+T細胞6 h,然後在體外以BALB/c小鼠異基因抗原分彆對其進行刺激,測定動員後的C57BL/6N小鼠脾細胞和na(i)ve CD4+T細胞的增殖能力以及上清液IL-4、IFN-γ的濃度,實驗分為G-CSF+IL-2組、G-CSF組、IL-2組和對照組.結果 G-CSF+IL-2組較列照組A值降低(P＜0.01);G-CSF+IL-2組IL-4水平高于對照組(P＜0.01),而IFN-γ水平低于對照組(P＜0.01).結論 rhG-CSF動員的C57BL/6N小鼠脾細胞和na(i)ve CD4+T細胞經IL-2預孵育後對同種異基因抗原免疫增殖能力明顯減弱併嚮Th2方嚮分化,兩者閤用對誘導T淋巴細胞免疫耐受有協同效應.
목적 탐토IL-2예부육대립세포집락자격인자(G-CSF)동원후적소서비림파세포공능적영향,종이위감경이기인조혈간세포이식시급성이식물항숙주병(GVHD)적발생제공일충신도경.방법 IL-2(50 U/ml)예부육rhG-CSF(매천0.25 μg/g)체내동원적C57BL/6N소서비세포화na(i)ve CD4+T세포6 h,연후재체외이BALB/c소서이기인항원분별대기진행자격,측정동원후적C57BL/6N소서비세포화na(i)ve CD4+T세포적증식능력이급상청액IL-4、IFN-γ적농도,실험분위G-CSF+IL-2조、G-CSF조、IL-2조화대조조.결과 G-CSF+IL-2조교렬조조A치강저(P＜0.01);G-CSF+IL-2조IL-4수평고우대조조(P＜0.01),이IFN-γ수평저우대조조(P＜0.01).결론 rhG-CSF동원적C57BL/6N소서비세포화na(i)ve CD4+T세포경IL-2예부육후대동충이기인항원면역증식능력명현감약병향Th2방향분화,량자합용대유도T림파세포면역내수유협동효응.
Objective To explore the influence of IL-2 pretreatment on splenic lymphocyte following mobilization with G-CSF, which may provide a new approach to attenuate acute graft-versus-host disease (GVHD). Methods Splenic cells and na(i)ve CD4+T cells from C57BL/6N mice receiving G-CSF-mobilized were pretreated with IL-2(50 U/ml) , and cocultured with the allogeneic antigens from BALB/c mice. The proliferation responses and the polarization of T cells were determined. C57BL/6N mice were randomly divided into 4 groups: G-CSF + IL-2 group, G-CSF group, IL-2 group , control group. Results Compared with the control group, IL-4 increased obviously while IFN-γ decreased significantly in the group of G-CSF + IL-2. The proliferation responses were also suppressed in vitro. Conclusion The proliferation responses of splenic cells and naive CD4 + T cells from C57BI/6N mice receiving G-CSF-mobilized to the allogeneic antigens were significantly abrogated by the pretreatment with IL-2, T cells were polarized toward the production of type-2 cytokines. The combination of G-CSF and IL-2 is potentially synergetic in the induction of T lymphocyte immune tolerance.
