CAJ | 학술논문

目的研究急性髓系白血病(acute myeloid leukemia,AML)中粘着斑激酶相关性鸟苷三磷酸酶调节因子(GTPase regulator associated with the focal adhesion kinase,GRAF)基因的表达及其临床意义.方法建立EvaGreen染料法实时定量聚合酶链反应(real-time quantitative PCR,RQ-PCR),对71例AML患者和21例良性血液病患者中GRAF转录本水平进行检测,分析其临床相关性.结果所建立的EvaGreen RQ-PCR方法具有良好的特异性、重复性,敏感度达102拷贝/μL.AML患者中GRAF转录本水平(0.01%～169.75%,中位3.82%)较对照组(14.49%～126.85%,中位56.04%)显著降低(P<0.05),GRAF基因表达量与患者性别、年龄、外周血白细胞计数、血红蛋白、血小板计数、FAB亚型、染色体核型分组均无相关性(P>0.05).结论 AML患者中GRAF基因表达下调,可能与其发病有关.
목적 연구급성수계백혈병(acute myeloid leukemia,AML)중점착반격매상관성조감삼린산매조절인자(GTPase regulator associated with the focal adhesion kinase,GRAF)기인적표체급기림상의의.방법 건립EvaGreen염료법실시정량취합매련반응(real-time quantitative PCR,RQ-PCR),대71례AML환자화21례량성혈액병환자중GRAF전록본수평진행검측,분석기림상상관성.결과 소건립적EvaGreen RQ-PCR방법구유량호적특이성、중복성,민감도체102고패/μL.AML환자중GRAF전록본수평(0.01%～169.75%,중위3.82%)교대조조(14.49%～126.85%,중위56.04%)현저강저(P<0.05),GRAF기인표체량여환자성별、년령、외주혈백세포계수、혈홍단백、혈소판계수、FAB아형、염색체핵형분조균무상관성(P>0.05).결론 AML환자중GRAF기인표체하조,가능여기발병유관.
Objective To quantify the expression level of GRAF gene in acute myeloid leukemia (AMD and" analyze its clinical significance. Methods The EvaGreen real-time quantitative polymerase chain reaction (RQ-PCR) assay was established and performed to measure the GRAF gene transcripts in 71 cases with AML and 21 with nonmalignant hematological diseases. The clinical correlation of GRAF expression was analyzed. Results The established EvaGreen RQ-PCR assay had good specificity, reproducibility and sensitivity. The GRAF expression level was significantly lower in AML (0. 01%-169.75%, median 3. 82%) than that in controls (14. 49%-126. 85% , median 56. 04%) (P<0.05). There was no correlation between the level of GRAF transcript and the sex, age, hematologic parameters, FAB subtypes and karyotypic groups (P>0. 05). Conclusion The GRAF gene was down-regulated in AML, which might play a role in the leukemogenesis.