CAJ | 학술논문

目的：进行板蓝根低极性流分及其亚流分的分离，并研究其免疫活性。方法：用快速低压干柱柱层析从板蓝根中分离出一个低极性流分及其一组亚流分；用化学发光法检测其对人多形核细胞化学发光的影响。结果：板蓝根低极性流分具有双向免疫活性，在低浓度时具有激活作用，在高浓度时具有抑制作用；而亚流分的免疫活性并未因其纯度的提高而相应提高。结论：板蓝根低极性流分及其亚流分含有抗炎成分。
목적：진행판람근저겁성류분급기아류분적분리，병연구기면역활성。방법：용쾌속저압간주주층석종판람근중분리출일개저겁성류분급기일조아류분；용화학발광법검측기대인다형핵세포화학발광적영향。결과：판람근저겁성류분구유쌍향면역활성，재저농도시구유격활작용，재고농도시구유억제작용；이아류분적면역활성병미인기순도적제고이상응제고。결론：판람근저겁성류분급기아류분함유항염성분。
AIM: To make isolation of a low-polar fraction and itssubfractions from Banlangen, dried root of Isatis tinctoria L, and study their immunocompetence. METHODS: A low-polar fraction and its subfractions were isolated from Banlangen with dry-column flash chromatography. It was tested that the effect of those fractions on chemiluminescence reaction of human polymorphonuclear cells. RESULTS: A two-way immunocompetence was found from those fractions. The effect was activation while the concentrations of those fractions were low, and it was inhibition while their concentrations were high. Immunocompetence of the subfractions did not increase while their degree of purity increased. CONCLUSION: There are some anti-inflamation components in a low-polar fraction and its subfractions from ‘Banlangen’.