CAJ | 학술논문

目的　研究获得具有红光区高吸收系数的新型光敏剂的结构及其光生物活性。方法　以氯化血红素为原料合成了中卟啉与次卟啉，通过Birch还原将其转变为二氢中卟啉和二氢次卟啉。以血卟啉衍生物(HpD)为阳性对照测定了所合成卟啉及其还原所得相应二氢卟啉对还原辅酶Ⅱ(NADPH)在重水中光氧化作用的敏化效应和对小鼠肉瘤180的光动力损伤作用。结果　还原所得二氢卟吩的光敏化效应和对小鼠肉瘤180的光动力损伤作用均高于其还原前的母体化合物及目前临床使用的光动力治癌药物HpD(均为P＜0.01)。结论　卟啉还原为二氢卟吩后提高了对红光的吸收，光敏化力与光动力作用增强，是发展光动力治癌新药的一种有效途径。
목적　연구획득구유홍광구고흡수계수적신형광민제적결구급기광생물활성。 방법　이록화혈홍소위원료합성료중계람여차계람，통과Birch환원장기전변위이경중계람화이경차계람。이혈계람연생물(HpD)위양성대조측정료소합성계람급기환원소득상응이경계람대환원보매Ⅱ(NADPH)재중수중광양화작용적민화효응화대소서육류180적광동력손상작용。 결과　환원소득이경계분적광민화효응화대소서육류180적광동력손상작용균고우기환원전적모체화합물급목전림상사용적광동력치암약물HpD(균위P＜0.01)。 결론　계람환원위이경계분후제고료대홍광적흡수，광민화력여광동력작용증강，시발전광동력치암신약적일충유효도경。
Objective　To explore the possibitlity to obtain new photosensitizers with high absorption coefficient in red band of the spectra. Methods　Mesoporphyrin and deuteroporphyrin were synthesized from hemin and transformed to their corresponding chlorins through Birch reduction. The sensitizing effects on photooxidation of NADPH in deuterium oxide and photodynamic effects on sarcoma 180 in mice of the chlorins synthesized and their parent porphyrins were compared with those of HpD. Results　The photosensitizing abilities and tumor-photodynamic effects of chlorins obtained by reduction of porphyrins were shown to be much higher than that of the parent porphyrins and hematoporphyrin derivative,a drug for tumor-photodynamic therapy presently used in clinics(P＜0.01). Conclusions　The reduction of porphyrins increses the absorption in red band of the spectra and consequently intensifies the photosensitizing ability and tumor-photodynamic effects. It may become an effective approach for development of new drug in tumor photodynamic therapy.