CAJ | 학술논문

研究了溶剂种类、浓度,提取溶剂比例、提取次数、时间、温度,萃取次数与温度,以及柱纯化和重结晶等条件对提取农杆菌中辅酶Q_(10)的影响;进行了最佳工艺优化,确立了生产工艺,并进行了中试验证.结果表明,最佳工艺条件为:用5倍的体积分数95%的乙醇在50℃浸提2遍,每遍1 h,获得提取液;提取液浓缩后经过提取液-甲醇-石油醚(三者体积比为1:2:3)的萃取体系室温萃取3遍,浓缩石油醚相后经过1步硅胶层析柱纯化,2或3步乙醇重结晶.中试结果表明,该工艺获得辅酶Q_(10)的纯度>99%,平均回收率>80%.
연구료용제충류、농도,제취용제비례、제취차수、시간、온도,췌취차수여온도,이급주순화화중결정등조건대제취농간균중보매Q_(10)적영향;진행료최가공예우화,학립료생산공예,병진행료중시험증.결과표명,최가공예조건위:용5배적체적분수95%적을순재50℃침제2편,매편1 h,획득제취액;제취액농축후경과제취액-갑순-석유미(삼자체적비위1:2:3)적췌취체계실온췌취3편,농축석유미상후경과1보규효층석주순화,2혹3보을순중결정.중시결과표명,해공예획득보매Q_(10)적순도>99%,평균회수솔>80%.
The process parameters of extraction CoQ10 from Agrobacterium were carefully investigated and then validated by pilot-scale test in this manuscript. The optimum process parameters were decided as follows: firstly, extracting twice (one hour each time) using 5X 95% enthanol at 50 ℃ followed by a concentrated step then extracting three times at room temperature using a extraction system ratio of 1 : 2 : 3 (concentrated solution : methanol : Petroleum ether) and finally concentrating Petroleum ether phase, through one step of Silica Gel-Alumina Column purification, two to three steps of recrystalization by enthanol to obtain the aim product. The pilot-scale test shown the process gives a purity of over 99%, percentage recovery over 80%.