CAJ | 학술논문

利用各种表面消毒方法对采自海南岛三个地区的胡椒大田植株的外植体进行消毒试验,由于内源性污染,除胡椒成熟种子外,其它各种大田外植体的表面消毒均未能成功.以胡椒成熟种子无菌萌发的实生苗茎尖作外植体,在1/2MS(MS或B5)+1.5 mg/L BA+0～0.2 mg/L IAA(或NAA)上可实现丛生芽增殖.茎尖水平或竖直接种方法显著影响茎尖的增殖;水平接种茎尖的生长和增殖效果优于竖直茎尖接种方式.茎尖增殖率随BA浓度的增加而提高,但BA浓度大于2.0 mg/L时会使苗芽的质量降低,愈伤组织产生严重,苗芽细小,抽出不明显,颜色发黄甚至变白.附加或不附加100 mg/L AdSO4对丛生芽增殖没有明显影响.生根培养基以1/2MS+1.0 mg/L IBA+0.5～1.0 mg/L IAA为最优,生根率可达100%;在细沙:土:椰糠(1:1:1)的基质中常规炼苗,成活率可达98%以上.液体纸桥法对胡椒种胚进行培养,在不附加任何生长调节物质的培养基(MS、B5或SH)上只产生单苗,而在附加不同种类和不同浓度的生长调节物质的培养基上则诱导形成愈伤组织,但未能实现分化;以胡椒无菌萌发的实生苗胚轴和叶片切段作外植体进行培养,较易诱导产生愈伤组织,但难以实现分化.
이용각충표면소독방법대채자해남도삼개지구적호초대전식주적외식체진행소독시험,유우내원성오염,제호초성숙충자외,기타각충대전외식체적표면소독균미능성공.이호초성숙충자무균맹발적실생묘경첨작외식체,재1/2MS(MS혹B5)+1.5 mg/L BA+0～0.2 mg/L IAA(혹NAA)상가실현총생아증식.경첨수평혹수직접충방법현저영향경첨적증식;수평접충경첨적생장화증식효과우우수직경첨접충방식.경첨증식솔수BA농도적증가이제고,단BA농도대우2.0 mg/L시회사묘아적질량강저,유상조직산생엄중,묘아세소,추출불명현,안색발황심지변백.부가혹불부가100 mg/L AdSO4대총생아증식몰유명현영향.생근배양기이1/2MS+1.0 mg/L IBA+0.5～1.0 mg/L IAA위최우,생근솔가체100%;재세사:토:야강(1:1:1)적기질중상규련묘,성활솔가체98%이상.액체지교법대호초충배진행배양,재불부가임하생장조절물질적배양기(MS、B5혹SH)상지산생단묘,이재부가불동충류화불동농도적생장조절물질적배양기상칙유도형성유상조직,단미능실현분화;이호초무균맹발적실생묘배축화협편절단작외식체진행배양,교역유도산생유상조직,단난이실현분화.
Experiments on surface-sterilization methods were carried out with various explants collected from field-grown black pepper (Piper nigrum Linn.)c.v.Daye(Lampong Type),extensively cultivated in China. Due to endogenous contaminants,contamination of all types of explants except for seeds was not yet effectively controlled. In vitro clonal propagation of black pepper with shoot tips excised from aseptic seedlings through multiple-shoot multiplication methods is successfully achieved. The best establishment and proliferation of shoot tips was obtained on 1/2MS(MS or Bs)basal medium supplemented with 1.5 mg/L BA and 0～0.2 mg/L IAA(or NAA). Excised microshoots were rooted in vitro on 1/2MS in the presence of 1.0 mg/L IBA and 0.5～1.0 mg/L IAA with the optimum rooting results. Plantlets had been successfully acclimatized and transferred to the greenhouse conditions. Complete plants were grown from mature and immature zygotic embryos of black pepper incubated on filter paper bridges in test tubes containing liquid SH(MS or B5)basal medium with not any growth regulators,and calli were induced with different combinations of auxins and cytokinins. Subculture of those calli onto the multiplication medium and differentiation medium led to browning and death finally,and no plant regeneration occurred. The morphogenetic potential of other explants such as leaf pieces and hypocotyl segments from aseptic seedlings was also investigated in vitro. Callus induction was relatively easy on MS(1/2MS,B5 or SH)basal medium fortified with a wide range of auxin-cytokinin combinations,but most attempts to regenerate plants from the calli were unsuccessful due to serious browning occurred during the subculture onto the multiplication medium and differentiation medium.