CAJ | 학술논문

目的确立一种快速、准确检测水溶性卟啉(TPPS4)的分析方法,从而进一步了解水溶性卟啉和牛血清白蛋白(BSA)之间相互作用的机制.方法采用电化学法对TPPS4的极谱伏安行为进行了研究,同时采用电化学法、荧光法和紫外法对TPPS4与BSA之间的相互作用分别进行了研究.3种方法相互辅证使得试验结果更加可靠.结果在底液NaH2PO4-Na2HPO4缓冲液(pH7.18)中,TPPS4在-0.70 V(vs ScE)处有一个稳定而灵敏的还原峰,其峰电流与TPPS4浓度在1.0×10-7～1.0×10-5mol·L-1有良好的线形关系(r2=0.998 3,0.999 3),检测限LOD为3.0×10-8mol·L-1.平均标准回收率为99.59%,精密度较好,RSD为0.56%(n=5).在NH4C1-NH3·H2O缓冲液(pH 9.05)中,实验结果表明BSA与TPPS4相互作用生成1:1的TPPS4-BSA超分子体系.另外,加入环糊精体系后,磺丁醚-β-CD(SBE-β-CD)和羟丙基-β-CD(HP-β-CD)均能促进TPPS4与BSA发生反应.结论建立了一种简单、快速、准确的水溶性卟啉四-(4-磺基苯)卟啉(TPPS4)的电化学分析方法.卟啉类药物被环糊精包合后更容易与人体内的蛋白质进行作用,环糊精在卟啉类药物的控制、释放中具有重要的作用和意义.
목적학립일충쾌속、준학검측수용성계람(TPPS4)적분석방법,종이진일보료해수용성계람화우혈청백단백(BSA)지간상호작용적궤제.방법채용전화학법대TPPS4적겁보복안행위진행료연구,동시채용전화학법、형광법화자외법대TPPS4여BSA지간적상호작용분별진행료연구.3충방법상호보증사득시험결과경가가고.결과재저액NaH2PO4-Na2HPO4완충액(pH7.18)중,TPPS4재-0.70 V(vs ScE)처유일개은정이령민적환원봉,기봉전류여TPPS4농도재1.0×10-7～1.0×10-5mol·L-1유량호적선형관계(r2=0.998 3,0.999 3),검측한LOD위3.0×10-8mol·L-1.평균표준회수솔위99.59%,정밀도교호,RSD위0.56%(n=5).재NH4C1-NH3·H2O완충액(pH 9.05)중,실험결과표명BSA여TPPS4상호작용생성1:1적TPPS4-BSA초분자체계.령외,가입배호정체계후,광정미-β-CD(SBE-β-CD)화간병기-β-CD(HP-β-CD)균능촉진TPPS4여BSA발생반응.결론건립료일충간단、쾌속、준학적수용성계람사-(4-광기분)계람(TPPS4)적전화학분석방법.계람류약물피배호정포합후경용역여인체내적단백질진행작용,배호정재계람류약물적공제、석방중구유중요적작용화의의.
Aim To establish a simple, rapid and accurate electroanalytical method for water soluble porphyrin meso-tetrakis-(4-sulfonatophenyl) porphyrin (TPPS4);to clarify the reaction between water soluble porphyrins and bovine serum albumin (BSA);and to determine the interaction of TPPS4 with BSA in the absence of presence of cyclodextrins (CDs) , separately. Methods Three methods including LSV,UV spectroscopy and fluorescence spectroscopy bad been employed to the relevant experiments. The way of employing three methods at the same time could make the experiment results more reliable. Results In the supporting electrolyte of NaH2 PO4-Na2 HPO4 ( pH 7. 18 ), a sensitive reduction peak of TPPS4 was found by linear sweep voltammetry (LSV), the peak potential (Ep) was-0. 70 V (vs SCE). The relationship between the second derivative peak of LSV (ip") and the concentration of TPPS4 was linear from 1.0 × 10-7mol·L-1 to 1.0 × 10-5mol· L-1, the square of correlation coefficients (r2) were 0. 998 3 and 0. 999 3, respectively. The relative standard deviation (RSD) was 0. 56% ( n = 5 ). The mean recovery of TPPS4 was 99.59％. In NH4C1-NH3· H2O buffers (pH 9.05), it was proved that BSA and TPPS4 could interact with each other and form 1:1 TPPS4-BSA supramolecular system. Moreover, the interaction between TPPS4 and BSA had been investigated by adding cyclodextrins (CDs). The interaction of TPPS4 with BSA was facilitated both by hydroxypropyl-β-CD (HP-3-CD) and sulforbutylether-β-CD (SBE-3-CD). Conclusion An electroanalytical method for TPPS4 has been established by LSV. The porphyrin drugs included by CDs could react with protein existing inside the human body easier. The consequences of this article also show that CDs will play important role in controlling and releasing the porphyrin drugs.