CAJ | 학술논문

水稻苗期低温白化突变是水稻在发育早期对低温胁迫的一种适应性,是一种受发育和温度控制的条件表达,它与其他水稻白化突变有本质的不同.本研究利用便携式叶绿素测量仪测定了白化时期植株的叶绿素含量和用透射电镜观察了叶绿体的结构变化.结果发现叶绿素平均含量仅为1.2(SPAD),而叶绿体也不能正常发育仅有囊泡状结构.通过与9311的正反交实验及子代的分离表现证明该性状受一个隐性核基因的控制.另外利用SSR分子标记技术将该基因定位在第8染色体上,两侧最近的SSR标记RM5068和RM3702分别距基因0.5～1.1 cM和4.9 cM,基因被定位在约6个cM的区间内.我们将该基因暂时命名为al12.
수도묘기저온백화돌변시수도재발육조기대저온협박적일충괄응성,시일충수발육화온도공제적조건표체,타여기타수도백화돌변유본질적불동.본연구이용편휴식협록소측량의측정료백화시기식주적협록소함량화용투사전경관찰료협록체적결구변화.결과발현협록소평균함량부위1.2(SPAD),이협록체야불능정상발육부유낭포상결구.통과여9311적정반교실험급자대적분리표현증명해성상수일개은성핵기인적공제.령외이용SSR분자표기기술장해기인정위재제8염색체상,량측최근적SSR표기RM5068화RM3702분별거기인0.5～1.1 cM화4.9 cM,기인피정위재약6개cM적구간내.아문장해기인잠시명명위al12.
Seedling albino mutation resistant to low temperature is an adaptability of rice (Oryza sativa L.) to cold. The mutant, a conditional expression controlled by development and temperature, differs from other albino mutants. The chlorophyll content of the mutant was measured using a portable chlorophyll meter, and the ultrastructure of the chloroplast was observed using a transmission electron microscope. Chlorophyll content was 1.2 SPAD, and the chloroplast did not develop, with only small vesicle-like structures. A segregation analysis of the reciprocal crosses between the albino mutation line with the rice line 9311demonstrated that the albino trait was controlled by a single recessive gene, which was flanked by SSR markers RM5068 and RM3702 on the short arm of chromosome 8 with a distance of 0.5-1.1 cM and 4.9 cM, respectively. This gene was mapped within a 6 cM interval region and was tentatively referred to as all2.