CAJ | 학술논문

目的探讨PPARγ激动剂罗格列酮(RGZ)对糖尿病(DM)大鼠肾病有否保护作用及其机制.方法正常饮食,高脂高糖+链脲佐菌素制备DM模型,将其随机分为RGZ大剂量干预组灌胃RGZ小剂量干预组和糖尿病非干预组.12周处死大鼠,取血、尿、肾标本,检测血糖、肾功能、肾脏指数(KI)及UA1b、尿α-MG、β-MG.电镜观察肾脏超微结构.通过图像分析软件检测PPARγ、α-SMA及Ⅰ型胶原在肾脏的表达水平.结果与糖尿病非干预组比较,大剂量RGZ干预组的血糖、KI、UA1b、α-MG、β-MG及α-SMA、Ⅰ型胶原的表达显著降低(P<0.05), PPARγ的表达显著增多(P<0.05).结论 RGZ大剂量干预可能对DM大鼠的肾病有保护作用.
목적 탐토PPARγ격동제라격렬동(RGZ)대당뇨병(DM)대서신병유부보호작용급기궤제.방법 정상음식,고지고당+련뇨좌균소제비DM모형,장기수궤분위RGZ대제량간예조관위RGZ소제량간예조화당뇨병비간예조.12주처사대서,취혈、뇨、신표본,검측혈당、신공능、신장지수(KI)급UA1b、뇨α-MG、β-MG.전경관찰신장초미결구.통과도상분석연건검측PPARγ、α-SMA급Ⅰ형효원재신장적표체수평.결과 여당뇨병비간예조비교,대제량RGZ간예조적혈당、KI、UA1b、α-MG、β-MG급α-SMA、Ⅰ형효원적표체현저강저(P<0.05), PPARγ적표체현저증다(P<0.05).결론 RGZ대제량간예가능대DM대서적신병유보호작용.
Objective To study whether peroxisome proliferator-activated receptor gamma(PPARγ)agonist rosiglitazone has protective effects on diabetic nephropathy and to explore its possible mechanism.Methods Ten SOD rats were regarded as control group.All rats with high-sucrose-high-fat diet were injected STZ(35 mg/kg)to induce diabetic rat model.Then diabetic rats were randomly assigned to 3 diabetic groups treated with a low dose and a high dose RGZ,and without RGZ.At 12 weeks,all rats were sacrificed and then a series of data were evaluated such as serum glucose,serum creatinine,blood urine nitrogen,the ratio of kidney weight to body weight,urinary albumin and urinary α-MG,β-MG.The structure of renal tissue was observed by electron microscope.The protein expressions of α-SMA,collagen I and PPARγ in kidney were examined by immunohistochemical stain.Results Serum glucose,urine albumin,α-MG,β-MG,the ratio of kidney weight to body weight,the expression of α-SMA and collagen I in kidney tissue were significantly lower(P<0.05)and the expression of PPARr was higher(P<0.05)in the high dose RGZ-treated group than in diabetic group without RGZ treatment.Conclusions The high dose of rosiglitazone has protective effects on diabetic nephropathy