CAJ | 학술논문

以过硫酸钾作为引发剂,丙烯酸为单体,在超声作用下原位聚合制备聚丙烯酸(PAA)修饰Ru纳米复合颗粒(Ru-PAA).修饰后的Ru-PAA复合纳米颗粒,不仅水溶性和稳定性得到改善,同时具有良好的生物相容性.研究了Ru-PAA复合纳米颗粒与蛋白质的相互作用,并利用散射光谱法考察了光散射强度与蛋白质浓度之间的关系.结果发现: 在最佳的实验条件下,BSA浓度为0.05～1.2 mg/L、HSA浓度为0.05～3 mg/L时,其浓度与光散射强度均有良好的线性关系,检出限均为0.01 mg/L.该方法用于实际样品测定,得到了令人满意的结果.
이과류산갑작위인발제,병희산위단체,재초성작용하원위취합제비취병희산(PAA)수식Ru납미복합과립(Ru-PAA).수식후적Ru-PAA복합납미과립,불부수용성화은정성득도개선,동시구유량호적생물상용성.연구료Ru-PAA복합납미과립여단백질적상호작용,병이용산사광보법고찰료광산사강도여단백질농도지간적관계.결과발현: 재최가적실험조건하,BSA농도위0.05～1.2 mg/L、HSA농도위0.05～3 mg/L시,기농도여광산사강도균유량호적선성관계,검출한균위0.01 mg/L.해방법용우실제양품측정,득도료령인만의적결과.
Poly-acrylic acid (PAA) modified nano-Ru particles have been prepared in situ by using potassium persulfate as initiator and acrylic acid (AA) as monomer under ultrasonic irradiation. The obtained composite nano-particles are water-soluble and biocompatible. The interaction between protein and Ru-PAA composite nano-particles were studied, and the reaction of the composite nano-particles with proteins resulted in an enhanced resonance light scattering (RLS). Under the optimal conditions, the RLS intensity is proportional to the concentration of proteins with the liner range of 0.05～1.2 mg/L for BSA and 0.05～3.0 mg/L for HSA, and the detection limits are both 0.01 mg/L. The developed method has been applied to the determination of protein in real-world samples with satisfactory results.