解剖学报
解剖學報
해부학보
ACTA ANATOMICA SINICA
2009年
6期
1005-1007
,共3页
ATP受体%降钙素基因相关肽%感觉纤维%咽黏膜%免疫荧光组织化学%大鼠
ATP受體%降鈣素基因相關肽%感覺纖維%嚥黏膜%免疫熒光組織化學%大鼠
ATP수체%강개소기인상관태%감각섬유%인점막%면역형광조직화학%대서
Purinergic receptor%Calcitonin gene-related peptide%Afferent fiber%Pharyngeal mucosa%Immunofluoresenthistochmistry%Rat
目的 研究大鼠咽黏膜内三磷酸腺苷(ATP)受体P2X_3阳性感觉纤维的分布,为探究ATP在咽黏膜感觉信号传导中的作用提供形态学依据. 方法 成年Wistar大鼠12只,应用免疫荧光组织化学双标技术结合激光扫描共焦显微镜. 结果 1.在咽黏膜各部位均可观察到P2X_3阳性感觉纤维,纤维分为两类:一类为串珠样游离神经纤维;另一类纤维在黏膜内发出分支并形成复杂的树枝状末梢,纤维分支在黏膜内形成神经丛.2.可见许多降钙素基因相关肽(CGRP)阳性纤维缠绕在P2X_3阳性树枝状末梢周围,并有少量的串珠样P2X_3阳性纤维与CGRP共存.3.在岩神经节内可观察到许多P2X_3或CGRP免疫阳性神经元,其中有少量神经元同时呈P2X_3/CGRP免疫阳性. 结论 大鼠咽黏膜内不同类型的感觉纤维均表达ATP受体P2X_3阳性,提示ATP可能与咽黏膜伤害性刺激和其他生理性刺激向中枢的传递有关.
目的 研究大鼠嚥黏膜內三燐痠腺苷(ATP)受體P2X_3暘性感覺纖維的分佈,為探究ATP在嚥黏膜感覺信號傳導中的作用提供形態學依據. 方法 成年Wistar大鼠12隻,應用免疫熒光組織化學雙標技術結閤激光掃描共焦顯微鏡. 結果 1.在嚥黏膜各部位均可觀察到P2X_3暘性感覺纖維,纖維分為兩類:一類為串珠樣遊離神經纖維;另一類纖維在黏膜內髮齣分支併形成複雜的樹枝狀末梢,纖維分支在黏膜內形成神經叢.2.可見許多降鈣素基因相關肽(CGRP)暘性纖維纏繞在P2X_3暘性樹枝狀末梢週圍,併有少量的串珠樣P2X_3暘性纖維與CGRP共存.3.在巖神經節內可觀察到許多P2X_3或CGRP免疫暘性神經元,其中有少量神經元同時呈P2X_3/CGRP免疫暘性. 結論 大鼠嚥黏膜內不同類型的感覺纖維均錶達ATP受體P2X_3暘性,提示ATP可能與嚥黏膜傷害性刺激和其他生理性刺激嚮中樞的傳遞有關.
목적 연구대서인점막내삼린산선감(ATP)수체P2X_3양성감각섬유적분포,위탐구ATP재인점막감각신호전도중적작용제공형태학의거. 방법 성년Wistar대서12지,응용면역형광조직화학쌍표기술결합격광소묘공초현미경. 결과 1.재인점막각부위균가관찰도P2X_3양성감각섬유,섬유분위량류:일류위천주양유리신경섬유;령일류섬유재점막내발출분지병형성복잡적수지상말소,섬유분지재점막내형성신경총.2.가견허다강개소기인상관태(CGRP)양성섬유전요재P2X_3양성수지상말소주위,병유소량적천주양P2X_3양성섬유여CGRP공존.3.재암신경절내가관찰도허다P2X_3혹CGRP면역양성신경원,기중유소량신경원동시정P2X_3/CGRP면역양성. 결론 대서인점막내불동류형적감각섬유균표체ATP수체P2X_3양성,제시ATP가능여인점막상해성자격화기타생이성자격향중추적전체유관.
Objective To study the distribution of purinergic P2X_3 receptor immunoreactive afferent fibers in the rat pharyngeal mucosa and provide morphological evidence for exploring the role of ATP in the signal transduction of sensory stimuli. Methods Twelve adult Wistar rats were used and immunofluorescenthistochemical double-labeling technique combined laser confocal scanning microscope was applied in the present study. Results 1.P2X_3 immunoreactive fibers were observed on sections from all parts of pharyngeal mucosa. Two types of positive fibers were found. One was free nerve fibers covered with many varicosities. Another one ramified in mucosa and showed complex arborization endings. Nerve plexus in mucosa were formed by P2X_3 immunoreactive fiber ramifications. 2.Most calcitonin gene-related peptide (CGRP) positive fibers intermingled with P2X_3 immunoreactive fiber arborizations. A few P2X_3 immunoreactive fibers were covered with many varicosities co-localized with CGRP. 3.In petrosal ganglion, most neurons were stained with P2X_3 or CGRP immunoreactivity and small number of P2X_3 immunoreactive neurons co-stained with CGRP. Conclusion These results indicated that the different types of afferent fibers in rat pharyngeal mucosa expressed purinergic P2X_3 receptor immunoreactivity and ATP might be related to nociceptive or physiological signals transduction in rat pharyngeal mucosa.
