安徽农业科学
安徽農業科學
안휘농업과학
JOURNAL OF ANHUI AGRICULTURAL SCIENCES
2010年
1期
51,57
,共2页
锦灯笼%NAA%诱导成活率
錦燈籠%NAA%誘導成活率
금등롱%NAA%유도성활솔
Franchet Groundcherry Calyx%NAA%Induced survival rate
[目的]为锦灯笼的快繁技术提供参考.[方法]以锦灯笼茎尖为外植体,NAA浓度分别为0.1、0.3、0.5 mg/L,研究不同浓度NAA对锦灯笼诱导成活率的影响.[结果]不同浓度NAA对锦灯笼诱导成活率的影响不同,以处理③(MS+NAA 0.5 mg/L +6-BA 0.3 mg/L+琼脂8 g/L+蔗糖30 g/L)的诱导效果最佳,诱导成活率达93.3%;其次为处理②(MS+NAA 0.3 mg/L +6-BA 0.3 mg/L+琼脂8 g/L+蔗糖30 g/L),诱导率为63.3%;处理①(MS+NAA 0.1 mg/L +6-BA 0.3 mg/L+琼脂8 g/L+蔗糖30 g/L)的诱导率最低,仅43.3%.[结论]处理③是最佳诱导分化培养基.
[目的]為錦燈籠的快繁技術提供參攷.[方法]以錦燈籠莖尖為外植體,NAA濃度分彆為0.1、0.3、0.5 mg/L,研究不同濃度NAA對錦燈籠誘導成活率的影響.[結果]不同濃度NAA對錦燈籠誘導成活率的影響不同,以處理③(MS+NAA 0.5 mg/L +6-BA 0.3 mg/L+瓊脂8 g/L+蔗糖30 g/L)的誘導效果最佳,誘導成活率達93.3%;其次為處理②(MS+NAA 0.3 mg/L +6-BA 0.3 mg/L+瓊脂8 g/L+蔗糖30 g/L),誘導率為63.3%;處理①(MS+NAA 0.1 mg/L +6-BA 0.3 mg/L+瓊脂8 g/L+蔗糖30 g/L)的誘導率最低,僅43.3%.[結論]處理③是最佳誘導分化培養基.
[목적]위금등롱적쾌번기술제공삼고.[방법]이금등롱경첨위외식체,NAA농도분별위0.1、0.3、0.5 mg/L,연구불동농도NAA대금등롱유도성활솔적영향.[결과]불동농도NAA대금등롱유도성활솔적영향불동,이처리③(MS+NAA 0.5 mg/L +6-BA 0.3 mg/L+경지8 g/L+자당30 g/L)적유도효과최가,유도성활솔체93.3%;기차위처리②(MS+NAA 0.3 mg/L +6-BA 0.3 mg/L+경지8 g/L+자당30 g/L),유도솔위63.3%;처리①(MS+NAA 0.1 mg/L +6-BA 0.3 mg/L+경지8 g/L+자당30 g/L)적유도솔최저,부43.3%.[결론]처리③시최가유도분화배양기.
[Objective] The study aimed to provide the references on rapid propagation technique of Franchet Groundcherry Calyx. [Method]With stem top of Franchet Groundcherry Calyx as explant, concentration of NAA was set at 0.1, 0.3, 0.5 mg/L, the effects of different NAA concentration on induced survival rate were studied. [Method] Different NAA concentration had different effect on induced survival rate. The effect of treatment ③ (MS+NAA 0.5 mg/L + 6-BA 0.3 mg/L + agar 8 g/L+ sucrose 30 g/L) was the best, and its induced survival rate reached 93.3%; secondly for treatment ②, its induced survival rate was 63.3%; treatment ① had the lowest induced survival rate, which was 43.3%.[Conclusion] Treatment ③ was the best inductive differential medium.
