CAJ | 학술논문

采用液相色谱(HPLC)结合蒸发光散射检测器(ELSD)对食品中的石蜡残留物进行了分析和检测.利用正相色谱柱对石蜡和非石蜡组分进行了分离,而无需对石蜡组分进行逐一分离.利用t检验,对构成石蜡的烷烃组分在ELSD检测器上的线性响应进行了显著性误差分析,结果显示,石蜡中烷烃组分具有相似的线性响应.以此为定量依据,实现了食品中石蜡含量的快速定量分析.并对HPLC-ELSD的检测和确证结果与GC-MS法进行了对比.方法的线性范围为10～500 mg/L,相关系数为0.9988;检出限为1 mg/L.以10, 50和100 mg/kg浓度水平添加石蜡时,其回收率在84.6%～105.4%之间,相对标准偏差为5.4%～7.2%.
채용액상색보(HPLC)결합증발광산사검측기(ELSD)대식품중적석사잔류물진행료분석화검측.이용정상색보주대석사화비석사조분진행료분리,이무수대석사조분진행축일분리.이용t검험,대구성석사적완경조분재ELSD검측기상적선성향응진행료현저성오차분석,결과현시,석사중완경조분구유상사적선성향응.이차위정량의거,실현료식품중석사함량적쾌속정량분석.병대HPLC-ELSD적검측화학증결과여GC-MS법진행료대비.방법적선성범위위10～500 mg/L,상관계수위0.9988;검출한위1 mg/L.이10, 50화100 mg/kg농도수평첨가석사시,기회수솔재84.6%～105.4%지간,상대표준편차위5.4%～7.2%.
A method was proposed for the separation and determination of paraffin waxes in food by HPLC-evaporative light scattering detection (ELSD). A normal-phase column was used to separate nonparaffinic and paraffinic materials without resolving the latter into individual components. The t-test method was adopted for the evaluation of mean difference between response factors of n-alkanes in paraffin waxes on ELSD detector. No mean difference was obtained between response factors, which can be used for quantitative determination of paraffin waxes in food. The determination results obtained by HPLC-ELSD were compared with those by GC-MS. The linear range for the determination of paraffin waxes was in the range from 10 to 500 mg/L with a correlation coefficient of 0.9988, and the limit of detection was 1.0 mg/L. With the spiking level of 10, 50 and 100 mg/kg, the recovery ranged from 84.6% to 105.4% and the relative standard deviation ranging from 5.4% to 7.2%. The proposed method is simple, fast and sensitive.