CAJ | 학술논문

目的探讨内皮素-1(ET-1)在烫(烧)伤大鼠脑血管损伤的表达及其作用.方法应用组织学方法观察8只烫伤大鼠脑基底动脉组织结构的变化;应用RT-PCR测定48只烫伤大鼠脑基底动脉ET-1 mRNA的表达;应用Western blotting分析48只烫伤大鼠脑基底动脉ET-1的表达;应用放射免疫分析检测48只烫伤大鼠脑基底动脉ET-1 水平的变化.结果烫伤大鼠脑基底动脉呈现病理学组织结构变化;烫伤后3h大鼠脑基底动脉ET-1 mRNA表达较正常对照组开始升高,6h 达高峰;烫伤后6h大鼠脑基底动脉ET-1 表达较正常对照组增强,12h 达高峰;烫伤后各组大鼠脑基底动脉ET-1水平较正常对照组明显升高,6h 达高峰.结论烫(烧)伤可诱发大鼠脑血管ET-1表达增加,增加表达的ET-1可能与烫(烧)伤大鼠脑血管损伤的病理发生有关.
목적 탐토내피소-1(ET-1)재탕(소)상대서뇌혈관손상적표체급기작용.방법 응용조직학방법관찰8지탕상대서뇌기저동맥조직결구적변화;응용RT-PCR측정48지탕상대서뇌기저동맥ET-1 mRNA적표체;응용Western blotting분석48지탕상대서뇌기저동맥ET-1적표체;응용방사면역분석검측48지탕상대서뇌기저동맥ET-1 수평적변화.결과 탕상대서뇌기저동맥정현병이학조직결구변화;탕상후3h대서뇌기저동맥ET-1 mRNA표체교정상대조조개시승고,6h 체고봉;탕상후6h대서뇌기저동맥ET-1 표체교정상대조조증강,12h 체고봉;탕상후각조대서뇌기저동맥ET-1수평교정상대조조명현승고,6h 체고봉.결론 탕(소)상가유발대서뇌혈관ET-1표체증가,증가표체적ET-1가능여탕(소)상대서뇌혈관손상적병리발생유관.
Objective To investigate the expression and effects of endothelin-1 (ET-1) in cerebral vessels of scalded rats. MethodHistological method was used to observe the alteration of histological structure of basilar artery in 8 scalded rats. Reverse transcription polymerase chain reaction(RT-PCR) was performed to examine expression of ET-1 mRNA;Western blotting to analyse expression of endothelin-1;radioimmunoassay to measure ET-1 levels;R3espectively, of cerebral basilar artery in 48 scalded rats. ResultVascular pathohistological changes were observed in cerebral basilar artery after rats were scalded;ET-1, ET-1 mRNA expression and ET-1 levels of basilar artery in scald 6 hours, 12 hours and 24 hours group were obviously higher than that of control group. Conclusion Scald may cause an increases of ET-1 expression in basilar artery, and elevated ET-1 expression may relate to the pathogenesis of cerebral vascular injury in scalded rats.